Miniaturized circulatory measuring chamber with integrated chemo- and/or biosensor elements

• US 5,846,392 A
• Filed: 10/08/1996
• Issued: 12/08/1998
• Est. Priority Date: 03/12/1994
• Status: Expired due to Fees

• Current Assignee: Knoll, Meinhard
• Original Assignee: Knoll, Meinhard
• Inventors: Knoll, Meinhard
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/702,563
• Time in Patent Office: 791 Days
• Field of Search: 204/403, 204/409, 422/82.02, 422/82.03
• US Class Current: 205/778
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

A miniaturized circulatory measuring chamber with integrated chemo- and/or biosensor elements including a substrate formed from a plate-shaped carrier. Within the plate-shaped carrier is inserted at least one containment tapering from a frontal surface of the plate-shaped carrier toward a second surface. At least one plate is connected to the second surface. At least one duct-shaped cavity is in contact with a small opening of the containment.

Claims

1. A miniaturized circulatory measuring chamber integrated with one of a chemosensor and a biosensor, comprising:

a carrier plate having a frontal surface and a second surface;

at least one containment formed in the carrier plate which tapers from the frontal surface to the second surface, the at least one containment having an opening;

a substance-recognition material within the at least one containment;

at least one supplementary plate connected to the second surface of the carrier plate; and

at least one duct located in a region of at least an interface between the carrier plate and the at least one supplementary plate, the at least one duct communicating with the opening of the at least one containment.

2. The miniaturized circulatory measuring chamber of claim 1, wherein the substance-recognition material forms an active sensor surface in a region of the at least one containment opening.

3. The miniaturized circulatory measuring chamber of claim 1, wherein the substance-recognition material comprises a substance-recognition membrane material, and wherein the at least one containment is closed by an encapsulating layer.

4. The miniaturized circulatory measuring chamber of claim 1, wherein the at least one duct is further located in the carrier plate.

5. The miniaturized circulatory measuring chamber of claim 1, wherein the at least one duct is further located in the at least one supplementary plate.

6. The miniaturized circulatory measuring chamber of claim 1, wherein the opening of the at least one containment forms a lower opening, and wherein the at least one containment also has an upper opening, and wherein a photo-linkable substance-recognition material is cross-linked by UV radiation after entering the at least one containment through one of the upper containment opening and the lower containment opening.

7. The miniaturized circulatory measuring chamber of claim 1, further comprising pumps, reaction paths, and valves for circulating a liquid measuring medium past the at least one containment.

8. The miniaturized circulatory measuring chamber of claim 1, wherein the substance-recognition material comprises a substance-recognition membrane material which is contained in the at least one containment and which is immobilized in an immobilizing material.

9. The miniaturized circulatory measuring chamber of claim 8, wherein the substance-recognition material is at least one member selected from the group consisting of enzymes and antibodies.

10. The miniaturized circulatory measuring chamber of claim 1, wherein the carrier plate comprises Si covered with an insulating layer, and wherein at least one electrical contact layer leads to the at least one containment, and wherein the substance-recognition material comprises a substance-recognition membrane material which is contained within the at least one containment.

11. The miniaturized circulatory measuring chamber of claim 10, wherein the at least one electrical contact layer comprises a film of noble metal.

12. The miniaturized circulatory measuring chamber of claim 11, wherein the noble metal is selected from the group consisting of platinum, gold, and silver.

13. The miniaturized circulatory measuring chamber of claim 10, wherein the at least one electrical contact layer comprises graphite.

14. A miniaturized circulatory measuring chamber integrated with one of a chemosensor and a biosensor, comprising:

a carrier plate having a frontal surface and a second surface, the carrier plate including;

at least one containment formed in the carrier plate which tapers from the frontal surface to the second surface, the at least one containment having an opening,

a substance-recognition material within the at least one containment, and

at least one duct recess communicating with the opening of the at least one containment; and

at least one supplementary plate connected to the second surface of the carrier plate such that the at least one supplementary plate and the at least one duct recess form at least one duct communicating with the opening of the at least one containment.

15. A method of using a miniaturized circulatory measuring chamber wherein the method comprises integrating the measuring chamber with at least one of a chemosensor and a biosensor including:

a carrier plate having a frontal surface and a second surface, at least one containment formed in the carrier plate which tapers from the frontal surface to the second surface, the at least one containment having an opening, a substance-recognition material associated with the at least one containment, at least one supplementary plate connected to the second surface of the carrier plate, and at least one duct located in a region between the carrier plate and the at least one supplementary plate, the duct communicating with the opening of the at least one containment;

wherein an analyte is measured in the duct depending upon the analyte and the substance-recognition material, by at least one of amperometric and/or potentiometric techniques.

16. The method of claim 15, wherein the measuring is performed in a flow-injection analysis arrangement.

17. A method for producing a miniaturized circulatory measuring chamber integrated with one of a chemosensor and a biosensor including:

a carrier plate having a frontal surface and a second surface, at least one containment formed in the carrier plate which tapers from the frontal surface to the second surface, the at least one containment having an opening and substance-recognition material having an active surface associated with the at least one containment, at least one supplementary plate connected to the second surface of the carrier plate, and at least one duct located in a region between the carrier plate and the at least one supplementary plate, the at least one duct communicating with the opening of the at least one containment, the method comprising;

forming the at least one containment in the carrier plate;

forming at least one duct recess in the second surface of the carrier plate by etching; and

covering the at least one duct recess by bonding the carrier plate to the at least one supplementary plate to form the at least one duct, such that a liquid measuring medium is capable of passing through the at least one duct and over the active surface of the substance-recognition material.

18. The method of claim 17, wherein the carrier plate comprises Si.

19. The method of claim 17, wherein the etching comprises anisotropic etching.

20. The method of claim 17, wherein the etching comprises isotropic etching.

21. The method of claim 17, wherein the at least one supplementary plate comprises a glass lid which is anodically bonded to the carrier plate.

22. The method of claim 17, wherein the at least one supplementary plate comprises a polymer film of a photostructurable dry resist laminated to the carrier plate.

23. The method of claim 17, wherein the at least one supplementary plate comprises a plastic film which is bonded to the carrier plate by adhesive.

24. The method of claim 17, further comprising forming metal film electrodes in the at least one duct by a thin-layer method.