Method of binding collagen recognizing substances to liposomes
First Claim
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1. A process for creating a bioadhesive liposome formed by covalently bonding a recognizing substance to a liposome comprising the steps of:
- (a) providing a reaction vessel containing a liposome having phosphatidylethanolamine;
(b) admixing a recognizing substance of collagen to the reaction vessel to form a reaction mixture with the liposome;
(c) buffering the reaction mixture;
(d) admixing a crosslinking reagent to the buffered reaction mixture;
(e) incubating the buffered reaction mixture and reagent for a period of time sufficient to form the covalent bonding of amine residues of the collagen recognizing substance and liposome to create a bioadhesive liposome; and
,(f) separating and removing excess unreacted and noncovalently bound materials from the bioadhesive liposome.
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Abstract
Recognizing substances, including epidermal growth factor, gelatin, collagen and hyaluronic acid, have been covalently bound to liposomal surfaces and utilized to attach liposomes onto a cellular or an extracellular matrix (ECM) target site. These "bioadhesive" liposomes offer several advantages including the mutual protection of both the drug and biological environment; an increase in drug bioavailability and retention at the target site; and improved adherence or adhesion to the designated target site.
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Citations
12 Claims
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1. A process for creating a bioadhesive liposome formed by covalently bonding a recognizing substance to a liposome comprising the steps of:
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(a) providing a reaction vessel containing a liposome having phosphatidylethanolamine; (b) admixing a recognizing substance of collagen to the reaction vessel to form a reaction mixture with the liposome; (c) buffering the reaction mixture; (d) admixing a crosslinking reagent to the buffered reaction mixture; (e) incubating the buffered reaction mixture and reagent for a period of time sufficient to form the covalent bonding of amine residues of the collagen recognizing substance and liposome to create a bioadhesive liposome; and
,(f) separating and removing excess unreacted and noncovalently bound materials from the bioadhesive liposome. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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- 8. A bioadhesive liposome designed for the sustained release of a therapeutic substance comprising a liposome component, the therapeutic substance encapsulated by the liposome component, and a collagen recognizing substance component bonded to the liposomal surface.
- 11. A modified liposome comprising a liposome having phosphatidylethanolamine as a provider of a primary amine residue covalently linked by a crosslinking reagent to a target recognizing substance which is collagen.
Specification