×

Reagent including an osmium-containing redox mediator

  • US 5,846,702 A
  • Filed: 12/20/1996
  • Issued: 12/08/1998
  • Est. Priority Date: 12/30/1993
  • Status: Expired due to Term
First Claim
Patent Images

1. A reagent for an electrochemical device that measures an analyte from a fluid sample, comprising:

  • a redox mediator and an enzyme, the redox mediator having the formula ##STR5## wherein R and R'"'"' are the same or different and are 2,2'"'"'-bipyridyl, 4,4'"'"'-disubstituted-2,2'"'"'-bipyridyl, 5,5'"'"'-disubstituted-2,2'"'"'-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, or 5,6-disubstituted-1,10-phenanthrolinyl, wherein the disubstitution is a methyl, ethyl, or phenyl group,R and R'"'"' are coordinated to Os at their nitrogen atoms,R" is hydrogen, methyl or ethyl,Z is chloro or bromo,m is +2,X is an anion and is chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, nitrate, sulfate, carbonate, or sulfite,Y is an anion and is chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate or nitrate, andn is 1 or zero,but when X is sulfate, carbonate, or sulfite, n is zero,wherein the aqueous solubility of the redox mediator is greater than about 1 millimolar, andwherein the redox mediator is reduced by a reaction involving the enzyme, the analyte, and the redox mediator, and the redox mediator is in sufficient amount such that oxidation of the reduced form of the redox mediator produces a current that correlates to the concentration of the analyte in the fluid sample, andthe enzyme is in sufficient amount to catalyze the reaction involving the enzyme, the analyte, and the redox mediator.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×