Processes for treating cellulosic material

• US 5,846,787 A
• Filed: 07/11/1994
• Issued: 12/08/1998
• Est. Priority Date: 07/11/1994
• Status: Expired due to Term

• Current Assignee: Purdue Research Foundation (Purdue University)
• Original Assignee: Purdue Research Foundation (Purdue University)
• Inventors: Westgate, Paul L., Kohlman, Karen L., Weil, Joseph R., Yang, Yiqi, Ladisch, Michael R.
• Primary Examiner(s): Marx, Irene
• Application Number:
  US08/273,417
• Time in Patent Office: 1,611 Days
• Field of Search: 435/209, 435/105, 435/99, 435/165, 435/163, 435/252
• US Class Current: 435/99
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Abstract

Disclosed are processes for pretreating cellulosic materials in liquid water by heating the materials in liquid water at a temperature at or above their glass transition temperature but not substantially exceeding 220° C., while maintaining the pH of the reaction medium in a range that avoids substantial autohydrolysis of the cellulosic materials. Such pretreatments minimize chemical changes to the cellulose while leading to physical changes which substantially increase susceptibility to hydrolysis in the presence of cellulase.

Claims

1. A process for treatment of a cellulose-containing material, comprising:

pretreating the cellulose-containing material to increase the susceptibility of the material to enzymatic hydrolysis by a cellulase enzyme, said pretreating including combining the material and liquid water in a reactor to form an aqueous pretreatment medium, and heating the aqueous pretreatment medium to a temperature of about 160°

C. to about 220°

C. while maintaining the pH of the treatment medium in the range of about 5 to about 8 by the addition of base; and

thereafter contacting the pretreated cellulosic material with a cellulase enzyme to enzymatically hydrolyze the cellulosic material.

2. The process of claim 1 wherein the cellulosic material is microcrystalline cellulose, and said process further comprises periodically adding base to the pretreatment medium to control its pH.

3. The process of claim 1 wherein the cellulosic material is or is derived from wood, oilseeds, cowpeas, corn, corn fiber, wheat, rice, barley, sugar cane, or cotton.

4. The process of claim 3 wherein the cellulosic material contains stems, siliques or leaves of Rapeseed plants.

5. The process of claim 3 wherein the cellulosic material contains Cowpea stems, leaves or pods.

6. The process of claim 3 wherein the cellulosic material contains Rice leaves or stems.

7. A process for enzymatically hydrolyzing a cellulosic material, comprising:

enzymatically hydrolyzing a pretreated cellulosic material in the presence of a cellulase enzyme;

said cellulosic material being the product of a pretreatment process in which the cellulosic material is heated in a liquid water medium to a temperature of about 160°

C. to about 220°

C. while maintaining the pH of the liquid water medium in the range of about 5 to about 8, so as to increase the susceptibility of the cellulosic material to hydrolysis by the cellulase enzyme.

8. The process of claim 7 wherein the cellulosic material is microcrystalline cellulose, and said pretreatment process further comprises periodically adding base to the pretreatment medium to control its pH.

9. The process of claim 7 wherein the cellulosic material is or contains components of wood, oilseeds, cowpeas, corn, corn fiber, wheat, rice, barley, sugar cane, or cotton.

10. The process of claim 7 wherein the cellulosic material contains stems, siliques or leaves of Rapeseed plants.

11. The process of claim 7 wherein the cellulosic material contains Cowpea stems, leaves or pods.

12. The process of claim 7 wherein the cellulosic material contains Rice leaves or stems.

13. A process for pretreating microcrystalline cellulose to increase its susceptibility to hydrolysis by a cellulase enzyme, and for hydrolyzing the pretreated microcrystalline cellulose, comprising:

pretreating microcrystalline cellulose by heating the microcrystalline cellulose in a liquid water medium at a temperature of about 160°

C. to about 220°

C.;

controlling the pH of the liquid water medium during the period that it is in said temperature range, so as to maintain the pH of the liquid water medium in the range of about 5 to about 8; and

recovering therefrom a pretreated microcrystalline cellulose having an increased susceptibility to hydrolysis by a cellulase enzyme; and

enzymatically hydrolyzing the pretreated microcrystalline cellulose in the presence of a cellulase enzyme.

14. The process of claim 13 wherein said controlling the pH includes adding base to the liquid water medium.

15. The process of claim 14 wherein the base is an alkali metal hydroxide, an alkaline earth metal hydroxide, or ammonium hydroxide.

16. The process of claim 15 wherein the base is sodium or potassium hydroxide.

17. The process of claim 15 wherein the base is ammonium hydroxide.

18. The process of claim 16 wherein the base is potassium hydroxide.

19. A process for hydrolyzing a crude plant cellulose material containing hemicellulose and lignin and which has been pretreated to increase the susceptibility of the cellulose material to hydrolysis by a cellulase enzyme, comprising:

hydrolyzing a pretreated cellulose material in the presence of a cellulase enzyme, wherein said pretreated cellulose material is the product of a process which comprises;

charging water and a crude plant cellulose material containing lignin and hemicellulose to a reactor to form a pretreatment mixture; and

heating the pretreatment mixture to a temperature of at least about 160°

C. but not exceeding about 220°

C., the pH of the pretreatment mixture during said heating being in the range of about 5 to about 8;

thereby forming said pretreated cellulose material having an increased susceptibility to hydrolysis by said cellulase enzyme.

20. The process of claim 19 wherein the cellulosic material contains stems, siliques or leaves of Rapeseed plants.

21. The process of claim 19 wherein the cellulosic material contains Cowpea stems, leaves or pods.

22. The process of claim 19 wherein the cellulosic material contains Rice leaves or stems.

23. A process for hydrolyzing a pretreated cellulosic material, comprising:

enzymatically hydrolyzing a pretreated cellulosic material in the presence of a cellulase enzyme, said pretreated cellulosic material being the product of a process which comprises;

providing a pretreatment medium containing a cellulosic material in water;

heating the pretreatment medium in a reaction vessel at a pressure at or above the saturation vapor pressure of water at the temperature of the heating, and while maintaining the pH of the medium at a value at which no substantial acid- or base-catalyzed autohydrolysis of the cellulosic material occurs; and

causing the pretreatment medium to cool and the pressure to dissipate so as to avoid explosive decompression of the cellulosic material;

so as to increase the susceptibility of the cellulosic material to cellulase-catalyzed hydrolysis.