Apparatus for biomass production

• US 5,846,816 A
• Filed: 07/10/1997
• Issued: 12/08/1998
• Est. Priority Date: 01/13/1995
• Status: Expired due to Fees

• Current Assignee: Enviro Research Pty Limited
• Original Assignee: Enviro Research Pty Limited
• Inventors: Forth, Kevin
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/860,818
• Time in Patent Office: 516 Days
• Field of Search: 435/286.7, 435/289.1, 435/292.1, 435/296.1, 435/304.1, 366/101, 366/107, 366/165.2
• US Class Current: 435/292.1
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/02 Form or structure of the vessel large containers B65D88/00
• CPC Class Codes: C12M 29/14 Pressurized fluid
• CPC Class Codes: C12M 31/08 by conducting or reflecting elements located inside the reactor or in its structure

Abstract

A bioreactor for biomass production having (i) a substantially transparent chamber, the chamber being suitable for containing biomass in a liquid phase, and having a base portion, an upper portion and a number of sidewalls between the base portion and the upper portion, the sidewalls being configured so as to diverge from the base portion towards the upper portion; and (ii) a circulating means for circulating the contents of the chamber to create a motive force in the liquid phase to ensure continual mixing of all of the biomass and a semi-continuous exposure of the biomass to a light source.

Claims

1. A bioreactor for biomass production comprising:

(i) a substantially transparent chamber, the chamber being at least suitable for containing biomass in a liquid phase, and having a base portion, an upper portion and a number of side walls between the base portion and the upper portion, the side walls being configured so as to generally diverge from the base portion towards the wider upper portion; and

(ii) a circulating means for circulating the contents of the chamber, wherein the circulating means creates a motive force within the liquid phase sufficient to ensure continual mixing of substantially all of the biomass and at least cyclical exposure of biomass to a light source.

2. A bioreactor according to claim 1 wherein the circulation rate within the chamber is at least slightly greater than a circulation rate that would lead to settling of the biomass within the bioreactor.

3. A bioreactor according to claim 1 wherein the circulating means is located within the bioreactor at or near the base portion.

4. A bioreactor according to claim 1 wherein the circulation means is a conduit extending through the length of the chamber which is located near the base portion of the chamber, said conduit being perforated and connected to a gas source such that in operation the passage of gas out of the conduit and into the chamber causes circulation of the chamber contents.

5. A bioreactor according to claim 1 in combination with a reflective base surface, said bioreactor being positioned above said surface.

7. A bioreactor according to claims 1 or 6 wherein the bioreactor chamber is an elongate trough-like prism, the prism having an inverted triangular cross-section, which can accommodate large volumes of liquid phase.

8. A bioreactor according claims 1 or 6 wherein the bioreactor includes a temperature control means for controlling the temperature of the liquid phases.

9. A bioreactor according to claims 1 or 6 wherein the bioreactor contains at least a baffle which is positioned in a fashion to further enhance the circulation of the contents of the chamber.

10. A bioreactor according to claims 1 or 6 wherein the bioreactor includes a pH control means for controlling the pH of the liquid phase.

11. A bioreactor according to claims 1 or 6 wherein the bioreactor comprises one or more light conduction means which promote effective distribution of light within the chamber.

12. A bioreactor according to claims 1 or 6 wherein that bioreactor contains one or more measuring means which are capable of detecting variations in nutrient levels.

13. A bioreactor according to claim 12 wherein the measuring means are connected to a computer control means which is capable of monitoring nutrient levels in the liquid phase and when necessary capable of introducing nutrients into the bioreactor chamber.

14. A bioreactor according to claims 1 or 6 wherein the bioreactor includes one or more jacketing means which are capable of at least partially covering the external surface of the bioreactor.

15. A bioreactor according to claims 1 or 6 wherein the bioreactor is in fluid communication with a separate vessel through which the liquid phase is diverted prior to its return to the bioreactor.

16. A bioreactor according to claim 15 wherein the separate vessel is a reservoir, increasing the volume of the system.

17. A bioreactor according to claim 15 wherein the separate vessel is a dark phase area which is used during high light intensities and growth conditions.

18. A bioreactor according to claim 15 wherein the separate vessel is a settling system from which biomass may be removed from the liquid phase.

19. A bioreactor according to claim 1 or 6, further comprising a means for controlling the internal physical and chemical environment of the bioreactor by one or more computer means which are able to introduce nutrients or chemicals or modify the light or temperature regime of the liquid phase in response to change in one or more of these conditions.

20. A bioreactor according to claims 1 or 6 wherein the liquid phase is pumped through a separate vessel containing an entrained bubble contactor, or other means for dissolving carbon dioxide or other gases into the liquid phase before being returning it to the bioreactor.

21. A bioreactor according to claim 23 wherein the liquid phase is also pumped through a vessel containing a temperature controlling means which is capable of varying the temperature in the liquid phase before being returned to the bioreactor.

22. A bioreactor according to claims 1 or 6 wherein the bioreactor includes a multiple of chambers all of which are interconnected.

23. A bioreactor according to claim 22 wherein one or more of the chambers are cloaked in darkness to aid propagation of the biomass.

24. A bioreactor according to claim 22 wherein the flow of the liquid phase between the chambers is controlled by a computer control means which is capable of monitoring the biomass density within each chamber and which is capable of transferring liquid phase between the chambers in response to changes in biomass density in each chamber.

25. A method of cultivating biomass comprising the step of cultivating biomass in a bioreactor according to claim 1 wherein that biomass is grown in a continuous production cycle wherein there is a steady feed of essential nutrients and liquid phase into the bioreactor as well as a continuous discharge of liquid phase containing cultured biomass from the bioreactor.

26. A bioreactor according to claim 1 or 6, further comprising a cover means sealed to the side walls.

27. A bioreactor according to claim 26, wherein the middle portion of the cover means is elevated relative to the junction between the cover means and the side walls.

28. A bioreactor according to claim 26 wherein the cover means is of an arcuate shape, with a generally angular upper surface.

29. A bioreactor according to claim 28, wherein the upper surface of the cover means is provided with one or more vents positioned towards the middle of the angular upper surface, which enables gas to be vented from the chamber, wherein the liquid phase within the bioreactor is maintained at a level which ensures contact of the liquid phase with the cover means.

30. A bioreactor according to claims 1,2,26,27,28, or 5, wherein the circulating means is located adjacent to the base portion within the bioreactor and is capable of generating an upward movement of biomass and liquid phase from the base portion to the top of the bioreactor whereupon the biomass impacts the top surface of the vessel allowing exposure to external light which may continue as the biomass then moves outwardly towards the sides of the bioreactor and then down the sides of the bioreactor towards the base portion, from where it is again caught by the circulation means and driven upwards towards the top of the bioreactor.

31. A bioreactor according to claim 29 wherein the vents contain a filter means which is capable of preventing particulate from entering the chamber.

6. A bioreactor for biomass production comprising:

(i) a substantially transparent chamber, the chamber being at least suitable for containing biomass in a liquid phase, and having a narrow base portion, an upper portion capable of being sealed by a cover means and a number of side walls diverging from the base portion to the wider upper portion; and

(ii) a circulation means for circulating the contents of the chamber, wherein the circulating means creates a motive force sufficient to ensure continual mixing of the liquid phase and exposure of biomass to the light wherein the circulating means is located at or near the lower most portion of the base portion and comprises a conduit extending through the length of the bioreactor, the conduit being perforated and connected to a gas source wherein the perforations in the conduit are disposed towards the underside of the conduit, such that in operation the downward passage of gas out of the conduit and into the liquid phase (a) prevents particulate material in the chamber from settling below the conduit, (b) causes circulation of the liquid phase; and

(c) allows gas diffusion into the liquid phase.