Sensor using long range surface plasmon resonance with diffraction double-grating

• US 5,846,843 A
• Filed: 11/18/1996
• Issued: 12/08/1998
• Est. Priority Date: 11/18/1996
• Status: Expired due to Fees

• Current Assignee: University of Toledo
• Original Assignee: University of Toledo
• Inventors: Simon, Henry John
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/751,614
• Time in Patent Office: 750 Days
• Field of Search: 385/12, 385/129, 385/130, 385/131, 422/55, 422/57, 422/82.05, 422/82.11, 435/287.1, 435/287.2, 435/288.7, 435/808, 436/164, 436/518, 436/524, 436/525, 436/527, 436/531, 436/535, 436/805
• US Class Current: 436/527
• CPC Class Codes: G01N 21/554 detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

A long range surface plasmon resonance sensor for use in biological, biochemical or chemical testing. The sensor includes a first dielectric medium and a second dielectric medium having an index of refraction approximately matching the first dielectric medium. A double-grating structure is located between the first dielectric medium and the second dielectric medium. A beam of electromagnetic radiation is introduced into the second dielectric medium in a manner which causes long range surface plasmon resonance to occur such that the beam of radiation suffers attenuated total reflection. The characteristics of the resonance dependent upon the reaction between the bonding layer and the targeted bonding molecule are then detected.

Claims

1. A long range surface plasmon resonance sensor for detecting molecules in a liquid solution comprising:

a first dielectric medium;

a second dielectric medium having an effective index of refraction approximately matching said first dielectric medium;

a double-grating structure between said first dielectric medium and said second dielectric medium;

the second dielectric medium including,

a first organic layer of molecules bound to said double-grating structure specific for said molecules that are to be detected,

means for introducing into the first dielectric medium a beam of electromagnetic radiation in a manner which causes long range surface plasmon resonance to occur whereby said beam of radiation suffers attenuated total reflection; and

a detector for detecting the attenuated total reflectance of the long range surface plasmon resonance as a function of the formation of a second organic layer of molecules on said first organic layer as the molecules in the liquid solution bind to the molecules of the first organic layer.

2. The long range surface plasmon resonance sensor of claim 1 wherein said first organic layer is a layer of antigen molecules and said second organic layer is a layer of antibody molecules.

3. The long range surface plasmon resonance sensor of claim 2 wherein said first dielectric medium is a material in which an electromagnetic wave can be propagated with a minimum dissipation of power.

4. The long range surface plasmon resonance sensor of claim 2 in which said first dielectric medium is a high refractive index glass or plastic material transparent to electromagnetic radiation.

5. The long range surface plasmon resonance sensor of claim 2 in which the refractive index of said first dielectric medium is about 1.4.

6. The long range surface plasmon resonance sensor of claim 2 in which the refractive index of said first dielectric medium matches that of the liquid solution containing the molecules to be detected.

7. The long range surface plasmon resonance sensor of claim 1 in which a grating surface is formed in the bottom surface of said first dielectric medium.

8. The long range surface plasmon resonance sensor of claim 1 in which said double-grating structure is an assembly of narrow grooves having inclined sidewalls.

9. The long range surface plasmon resonance sensor of claim 1 in which said double-grating structure is in the form of a compact disk.

10. The long range surface plasmon resonance sensor of claim 1 in which said double-grating structure is a metal film of uniform thickness.

11. The long range surface plasmon resonance sensor of claim 1 in which said double-grating structure is a metal film no greater than about 20 nm thick.

12. The long range surface plasmon resonance sensor of claim 11 in which said metal film is selected from the group consisting of silver, gold and aluminum.

13. The long range surface plasmon resonance sensor of claim 1 in which said liquid solution is a serum containing antibody proteins.

14. The long range surface plasmon sensor of claim 2 wherein said antigen molecules of said first organic layer are a macromolecular layer of uniform thickness.

15. The long range surface plasmon resonance sensor of claim 2 wherein the attenuated total reflectance of the long range surface plasmon resonance are caused by attachment of ligands in said first organic layer to said second organic layer.

16. The long range surface plasmon resonance sensor of claim 15 wherein the ligands are bifunctional or polyfunctional molecules consisting of at least one portion of an antigen complimentary to an antibody of said second organic layer.

17. The long range surface plasmon resonance sensor or claim 16 wherein the radiation source produces a collimated input beam of electromagnetic radiation having a frequency to generate surface plasmon waves.

18. A long range surface plasmon resonance sensor for detecting molecules in a liquid solution comprising:

a first dielectric medium;

a second dielectric medium having an effective index of refraction approximately matching said first dielectric medium;

a double-grating structure including a metal film no greater than about 20 nm thick between said first dielectric medium and said second dielectric medium;

the second dielectric medium including,

a first organic layer of molecules bound to the metal film specific for said molecules that are to be detected,

means for introducing into the first dielectric medium a beam of electromagnetic radiation in a manner which causes long range surface plasmon resonance to occur whereby said beam of radiation suffers attenuated total reflection; and

a detector for detecting the attentuated total reflectance of the long range surface plasmon resonance as a function of the formation of a second organic layer of molecules on said first organic layer as the molecules in the liquid solution bind to the molecules of the first organic layer.

19. The long range surface plasmon resonance sensor of claim 18 in which said first dielectric medium is a high refractive index glass or plastic material transparent to electromagnetic radiation.

20. A long range surface plasmon resonance sensor for detecting molecules in a liquid solution comprising:

a first dielectric medium;

a second dielectric medium having an effective index of refraction approximately matching said first dielectric medium;

said second dielectric medium including,

a first organic layer of molecules specific for said molecules that are to be detected,

a double-grating structure including a metal film no greater than about 20 nm thick between said first dielectric medium and said second dielectric medium;

means for introducing into the first dielectric medium a beam of electromagnetic radiation in a manner which causes long range surface plasmon resonance to occur whereby said beam of radiation suffers attentuated total reflection; and

a detector for detecting the attenuated total reflectance of the long range surface plasmon resonance as a function of the formation of a second organic layer of molecules on said first organic layer as the molecules in the liquid solution bind to the molecules of the first organic layer.