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Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system

  • US 5,849,489 A
  • Filed: 08/23/1996
  • Issued: 12/15/1998
  • Est. Priority Date: 11/07/1991
  • Status: Expired due to Fees
First Claim
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1. A biosensor for detecting the presence of an analyte in solution, said analyte comprising a target DNA sequence, said biosensor comprising:

  • an excitation source for delivering emitting photonic energy;

    a donor sequence comprising a first polynucleotide having a terminal donor chromophore and at least one intermediate donor chromophore linked to said first polynucleotide by linker arms, wherein said first polynucleotide is complementary to a first region of said target DNA sequence;

    an acceptor sequence comprising a second polynucleotide having at least one acceptor chromophore linked to said second polynucleotide by linker arms, wherein said second polynucleotide is complementary to a second region of said target DNA sequence; and

    an associated photon sensing means to detect photonic energy emitted from said acceptor;

    wherein said first polynucleotide and said second polynucleotide are able to hybridize with said target DNA sequence to form a complex wherein the distance between said terminal donor chromophore and one said acceptor chromophore is greater than 5 nm and there is at least one said intermediate donor chromophore.

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