Hepatitis G virus and molecular cloning thereof
First Claim
1. A vector comprising a Non-A Non-B Non-C Non-D Non-E Hepatitis Virus (HGV) polynucleotide composed of a contiguous series of nucleotides contained in SEQ ID NO:
- 14 operably linked to a control sequence, where said polynucleotide encodes a product at least 10 amino acids in length,wherein HGV is characterized by;
(i) production of elevated serum alanine aminotransferase levels in an infected primate, (ii) its serological distinction from hepatitis virus (HAV) hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus, and hepatitis E virus (HEV), (iii) membership in the virus family Flaviviridae and (iv) a viral genome comprising a polynucleotide region that is selectively hybridizable with SEQ ID NO;
19.
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Accused Products
Abstract
Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.
18 Citations
7 Claims
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1. A vector comprising a Non-A Non-B Non-C Non-D Non-E Hepatitis Virus (HGV) polynucleotide composed of a contiguous series of nucleotides contained in SEQ ID NO:
- 14 operably linked to a control sequence, where said polynucleotide encodes a product at least 10 amino acids in length,
wherein HGV is characterized by;
(i) production of elevated serum alanine aminotransferase levels in an infected primate, (ii) its serological distinction from hepatitis virus (HAV) hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus, and hepatitis E virus (HEV), (iii) membership in the virus family Flaviviridae and (iv) a viral genome comprising a polynucleotide region that is selectively hybridizable with SEQ ID NO;
19. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- 14 operably linked to a control sequence, where said polynucleotide encodes a product at least 10 amino acids in length,
Specification