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Amplification and detection process

  • US 5,849,544 A
  • Filed: 01/24/1995
  • Issued: 12/15/1998
  • Est. Priority Date: 07/24/1992
  • Status: Expired due to Term
First Claim
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1. A method for detecting a target nucleic acid sequence, which method involves amplification and detection in the same reaction vessel and comprises:

  • (a) amplification of the target nucleic acid sequence in a vessel which is provided with a solid phase capture probe comprising a nucleic acid sequence which hybridises to at least a portion of the said amplified target nucleic acid sequence, said capture probe not participating in nucleic acid sequence amplification processes, and said vessel being thermally stable, chemically derivatizable and thin-walled;

    (b) bringing a sample suspected of comprising said amplified target nucleic acid sequence into contact with said capture probe under conditions which allow said amplified target nucleic acid sequence to be bound by said capture probe; and

    (c) detecting the presence of bound amplified target nucleic acid sequence.

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