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Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers

  • US 5,849,878 A
  • Filed: 06/07/1995
  • Issued: 12/15/1998
  • Est. Priority Date: 08/13/1993
  • Status: Expired due to Term
First Claim
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1. A method for preparing a double stranded DNA-protein conjugate comprising(a) preparing a first thiolated single stranded DNA, wherein said first thiolated single stranded DNA is(1) about 10 to 100 bases in length, and(2) thiolated at either the 3'"'"' or 5'"'"' end;

  • (b) preparing a second thiolated single stranded DNA, said first and second thiolated single DNA strand being complementary to each other, and wherein said second thiolated single stranded DNA is(1) about 10 to 100 bases in length, and(2) thiolated at the same end as said first thiolated DNA strand;

    (c) reacting said first thiolated DNA strand with a thionitrobenzoate derivatized ligand binding first protein to form a first single stranded DNA-protein conjugate;

    (d) reacting said second thiolated DNA strand with a thionitrobenzoate derivatized ligand binding second protein to form a second single stranded DNA-protein conjugate;

    (e) annealing said first and second single stranded conjugates; and

    (f) recovering the double stranded DNA-protein conjugate product of step (e).

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