Bone morphogenetic protein (BMP)--6
First Claim
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1. A purified human bone morphogenetic protein-6 produced by the steps of(a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the DNA sequence of FIG. 3 from nucleotide 1321 to 1698;
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Abstract
Purified bone morphogenetic protein (BMP)-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
50 Citations
15 Claims
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1. A purified human bone morphogenetic protein-6 produced by the steps of
(a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the DNA sequence of FIG. 3 from nucleotide 1321 to 1698; - and
(b) recovering from said culture medium a protein comprising the amino acid sequence from amino acid 388 to amino acid 513 as shown in FIG. 3.
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2. A composition comprising a purified BMP-6! bone morphogenetic protein-6 produced by the steps of
(a) transforming a host cell with a DNA sequence comprising nucleotides #160 to #1712 of FIG. 3; -
(b) culturing said transformed cell in a suitable culture medium; and (c) isolating and purifying said protein from said culture medium. - View Dependent Claims (3, 4)
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5. A purified bone morphogenetic protein-6 (BMP-6) comprising an amino acid sequence selected from the group consisting of:
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(a) amino acids 412 through 513 of FIG. 3; (b) amino acids 388 through 513 of FIG. 3; and (c) amino acids 382 through 513 of FIG. 3. - View Dependent Claims (6, 7, 8, 9, 10)
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11. A BMP-6 protein encoded by a DNA sequence comprising a DNA sequence selected from the group consisting of the DNA molecules of ATCC deposits 68245 and 68021.
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12. A BMP-6 protein comprising amino acids 382 through 513 of FIG. 3.
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13. A purified BMP-6 polypeptide comprising an amino acid sequence encoded by a DNA sequence comprising a DNA sequence selected from the group consisting of:
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(a) nucleotides 1393 through 1698 of FIG. 3; (b) nucleotides 1321 through 1698 of FIG. 3; (c) nucleotides 1303 through 1698 of FIG. 3; and (d) naturally occurring allelic sequences and degenerative codon sequences of (a) through (c).
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14. A purified BMP-6 polypeptide encoded by a DNA sequence which hybridizes under stringent wash conditions of 0.1×
- SSC at 65°
C. to a DNA sequence selected from the group consisting of;(a) nucleotides 1393 through 1698 of FIG. 3; (b) nucleotides 1321 through 1698 of FIG. 3; (c) nucleotides 1303 through 1698 of FIG. 3; and (d) naturally occurring allelic sequences and degenerative codon sequences of (a) through (c).
- SSC at 65°
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15. A purified BMP-6 polypeptide comprising an amino acid sequence selected from the group consisting of amino acid sequences encoded by a DNA sequence comprising nucleotides 289 through 666 of FIG. 2.
Specification