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Isolation of lactoferrin from milk

  • US 5,849,885 A
  • Filed: 06/05/1995
  • Issued: 12/15/1998
  • Est. Priority Date: 02/16/1994
  • Status: Expired due to Term
First Claim
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1. A method for substantially purifying human lactoferrin from transgenic milk, comprising the steps of:

  • contacting transgenic bovine milk or milk fraction containing human lactoferrin and an endogenous lactoferrin with a strong cation exchange resin under elevated ionic strength conditions which are at least 10 mM higher in monovalent cation concentration than milk for a suitable binding period, forming human lactoferrin-resin complexes;

    removing the unbound milk or milk fraction components from the human lactoferrin-resin complexes; and

    eluting the human lactoferrin from the recovered human lactoferrin-resin complexes with a salt solution having an ionic strength sufficient to elute the human lactoferrin from the human lactoferrin-resin complexes whereupon human lactoferrin substantially free from the endogenous lactoferrin is obtained.

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