Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants

• US 5,850,019 A
• Filed: 08/06/1996
• Issued: 12/15/1998
• Est. Priority Date: 08/06/1996
• Status: Expired due to Term

• Current Assignee: University Of Kentucky Research Foundation (University of Kentucky)
• Original Assignee: University Of Kentucky Research Foundation (University of Kentucky)
• Inventors: Shepperd, Robert J., Maiti, Indu B.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): NELSON, AMY J
• Application Number:
  US08/692,511
• Time in Patent Office: 861 Days
• Field of Search: 536/24.1, 536/23.1, 536/23.2, 435/320.1, 435/410, 435/414, 435/419, 800/205, 800/250, 800/DIG. 9, 800/DIG. 13, 800/DIG. 17, 800/DIG. 24, 800/DIG. 26, 800/DIG. 27, 800/DIG. 44, 800/DIG. 42 , 800/DIG. 14, 800/DIG. 43, 800/DIG. 56, 800/DIG. 57, 800/DIG. 58, 800/DIG. 65, 800/DIG. 69, 800/DIG. 63
• US Class Current: 800/317.3
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells
• CPC Class Codes: C12N 2730/00022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The isolation, modification and use of wild-type and modified viral FLt promoters of peanut chlorotic streak caulimovirus (PClSV) in the expression of chimeric genes in plant cells. The FLt promoter from PClSV has been modified to have duplicated enhancer domains.

Claims

1. A DNA construct comprising a full-length transcript promoter from peanut chlorotic streak virus, at least one enhancer domain from peanut chlorotic streak virus, a heterologous gene and border regions from Agrobacterium tumefaciens, wherein said promoter is operably linked to said heterologous gene.

2. The DNA construct of claim 1, wherein said at least one enhancer domain is a single enhancer domain.

3. The DNA construct of claim 1, wherein said at least one enhancer domain is a double enhancer domain.

4. The DNA construct of claim 1, wherein said promoter comprises the nucleotide sequence of SEQ ID NO:

1.

5. The DNA construct of claim 1, having the designation pKLP6 or pKLP36, which is transformed in Escherichia coli and deposited as Accession No. NRRL B-21604 or Accession No. NRRL B-21605.

6. The DNA construct of claim 1, further comprising a non-translated leader sequence from peanut chlorotic streak virus.

7. A microorganism transformed with the DNA construct of claim 1.

8. The microorganism of claim 7, which is Escherichia coli.

9. The microorganism of claim 8, having Accession No. NRRL B-21604 or Accession No. NRRL B-21605.

10. A plant cell transformed with the DNA construct of claim 1.

11. The plant cell of claim 10, wherein said at least one enhancer domain of said DNA construct is a double enhancer domain.

12. The plant cell of claim 10, which is a cell from a plant selected from the group consisting of cotton, soybean, alfalfa, oilseed rape, flax, tomato, sugar beet, sunflower, potato, tobacco, maize, wheat, rice, lettuce and banana.

13. The plant cell of claim 2, which is a tobacco cell.

14. The plant cell of claim 10, which is from a tissue selected from the group consisting of calyx, filament, pedicel, style, ovary, corolla, anther, stigma, leaf, seed, embryo, stem and root.

15. A plant transformed with the DNA construct of claim 1.

16. The plant of claim 15, which is selected from the group consisting of cotton, soybean, alfalfa, oilseed rape, flax, tomato, sugar beet, sunflower, potato, tobacco, maize, wheat, rice, lettuce and banana.

17. The plant of claim 16 which is tobacco.