Specimen slide for a microscope

• US 5,851,489 A
• Filed: 11/15/1996
• Issued: 12/22/1998
• Est. Priority Date: 05/17/1994
• Status: Expired due to Term

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V., Bionas GmbH (HN Holding GmbH)
• Original Assignee: Micronas Intermetall GmbH (TDK Corporation)
• Inventors: Wolf, Bernhard, Baumann, Werner, Dumbs, Alfred, Sulz, Gerd, Sieben, Ulrich
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/737,688
• Time in Patent Office: 767 Days
• Field of Search: 422/82.01-82.02, 435/288.3, 435/287.9, 435/305.1
• US Class Current: 422/82.02
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/163 Biocompatibility
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

A specimen slide (1) for a microscope, camera or other such observation device has a receiving area (2) for cell or tissue samples or other such organic material and a smaller observation area (3) for the organic material. The specimen slide (1) at least in the observation area is made of transparent material. Within the receiving area (2) the specimen slide (1) is provided with thin-film sensors (6 to 12) adjacent to the observation area (3) for measuring physiological parameters of the organic material. The chemical and physical characteristics of the organic material under observation can be picked up by the sensors while it is observed, for example under a microscope. Sensors of differing types can be provided, such as interdigital capacitors (6), NO sensors (8), O₂ sensors (9) or temperature sensors (11), thereby making available important physiological measurement and/or control parameters.

Claims

1. A specimen slide for a microscope, camera or other such device for observing samples of living cells, tissue or other organic material;

the specimen slide (1) comprising;

a silicon chip having a receiving area (2) for said samples,

a smaller observation area (3) in the receiving area (2),

an opening in the silicon chip in the observation area (3), said opening having a window (26) of optically transparent material inserted therein for visually observing the samples,

a plurality of sensors (6 to

     12) integrated into the silicon chip in the receiving area (2) and distributed around the periphery of the observation area (3),

at least one of said sensors comprising a thin-film sensor or planer sensor for measuring physiological parameters of the samples, and

the receiving area having a structural surface adapted for cultivation of non-adherent cells.

2. A specimen slide as claimed in claim 1, characterized in that the sensors (6 to 12) are at least partly sunk in the surface of the specimen side (1).

3. A specimen slide as claimed in claim 1, characterized in that at least one sensor (6 to 12) takes the form of an integrated circuit with field-effect transistors.

4. A specimen slide as claimed in claim 1, characterized in that the surface of the specimen slide (1) is provided with strip conductors (13) which are printed, vapour-deposited and/or at least partly sunk in the surface.

5. A specimen slide as claimed in claim 1, characterized in that the sensors (6 to 12) are shielded against light and that for this purpose a layer impervious to light is provided.

6. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) has a microprocessor for processing and/or evaluating the sensor signals.

7. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) is temperature-regulable.

8. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) has a temperature-controlled thin-film heating and that the same is preferably provided on the specimen slide (1) at an outer surface thereof facing away from the receiving area (2).

9. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) is integrated in or connected to a standardized dual-in-line integrated circuit package.

10. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) essentially takes the form of a rectangular wafer and that the receiving area (2) is provided on one face (4) of the wafer.

11. A specimen slide as claimed in claim 1, characterized in that at least one locating recess (34) for inserting a contact member is provided on the specimen slide (1) adjacent to the narrow edges.

12. A specimen slide as claimed in claim 1, characterized in that the surface structure has a lamellar or lattice structure.

13. A specimen slide as claimed in claim 1, characterized in that the specimen slide (1) is provided with electric connections or contact surfaces (5) for detecting measured values of physiological properties, for measuring signals from the sensors and/or for supplying power to the sensors (6 to 12).

14. A specimen slide as claimed in claim 13, characterized in that for detecting electric signals and/or for feeding current, a contact member (33) which is adapted to be attached or pressed onto the contact surfaces (5) of the specimen slide (1) and has contacts mating the contact surfaces (5) of the specimen slide (1) is provided.

15. A specimen slide as claimed in claim 1, characterized in that a holder (14) is provided for fixing the specimen slide (1).

16. A specimen slide as claimed in claim 15, characterized in that the holder (14) is temperature-regulable and in the position of use is connected with good thermal conductivity to the specimen slide (1).

17. A specimen slide as claimed in claim 15, characterized in that the holder (14) has a liquid duct (15) or a hollow wall for temperature-regulating liquid, with a liquid inlet (16) and a liquid outlet (17).

18. A specimen slide as claimed in claim 15, characterized in that the holder (14) has a thermostatically controlled electric heating.

19. A specimen slide as claimed in claim 1, characterized in that a chamber closure (23) is provided laterally tightly surrounding the receiving area (2) of the specimen slide (1) and therewith defining a substantially closed cultivation chamber (27), that the chamber closure (23) has a viewing and/or illuminating window (26) and at least one inlet opening (28) and at least one outlet opening (29) for a nutrient liquid.

20. A specimen slide as claimed in claim 19, characterized in that the window (26) is integral with the chamber closure (23) and of comprises biologically inert, highly transparent plastic.

21. A specimen slide as claimed in claim 19, characterized in that the window is arranged in a depression provided in the upper surface of the chamber closure (23).

22. A specimen slide as claimed in claim 19, characterized in that thin-film sensors or planar sensors are provided on a chamber closure (23) wall facing the chamber (27).

23. A specimen slide as claimed in claim 19, characterized in that the specimen slide (1) has running round the periphery of the receiving area (2) a holding edge (22) which projects upwardly and surrounds an opening extending to the receiving area (2), and that the chamber closure (23) is adapted to be detachably inserted in the opening.

24. A specimen slide as claimed in claim 19, characterized in that the chamber closure (23) has locating openings (31) into which additional sensors (32) with sensor holders can be detachably placed.

25. A specimen slide as claimed in claim 24, characterized in that stoppers are provided for closing off locating openings (31) not required.