Multiparameter hematology apparatus and method

• US 5,851,835 A
• Filed: 12/12/1996
• Issued: 12/22/1998
• Est. Priority Date: 12/18/1995
• Status: Expired due to Fees

• Current Assignee: Laboratory Technologies Corporation
• Original Assignee: Laboratory Technologies Corporation
• Inventors: Groner, Warren
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/764,322
• Time in Patent Office: 740 Days
• Field of Search: 436/63, 436/66, 436/69, 436/70, 436/164, 436/165, 422/73, 422/82.05, 422/82.09, 435/2, 382/133, 382/134, 356/39, 356/40, 356/300, 356/311, 356/317, 356/318, 356/336, 356/337
• US Class Current: 436/63
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 15/0227 using imaging; using holography
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80

Abstract

A multi-parameter hematology analyzer and method are provided for in vitro determination of blood parameters. The analyzer apparatus includes a laser for transmitting light through a specimen container containing a minimally diluted blood sample. The light scattered by the constituents of the blood sample are scattered into a pattern which is recorded on a camera image. This image is then processed by image processing software and is used to establish specific blood parameters.

Claims

1. A multiparamenter hematology apparatus comprising, in combination:

a source of light energy configured to emit laser light in a red spectrum;

a reservoir for containing a blood specimen diluted to less than 1/100 the reservoir having a transparent portion in line with the laser light emitted from the source of light energy and providing access to an ensemble light scattering pattern from the blood specimen;

a camera disposed in line with the transparent portion for receiving the ensemble light scattering pattern of the laser light from an ensemble of blood cells contained in the specimen and providing images thereof;

a digitizing means for digitizing the images, in operative communication with the camera for receiving frames of images of the ensemble light scattering pattern and converting at least a portion thereof into digital images; and

a processor connected to the digitizing means for performing image analysis on the digital images of the ensemble light scattering pattern to establish blood parameters therefrom including intrinsic parameters such as cell size, extrinsic parameters such as cell counts as well as aggregation and sedimentation of blood cells.

2. The apparatus of claim 1, wherein the source of light is selected from the group consisting of a He Ne laser and a diode laser.

3. The apparatus of claim 1, wherein the reservoir for containing a blood specimen is a transparent specimen container configured to define a path width therethrough of from about 0.1 mm to about 0.5 mm.

4. The apparatus of claim 1, wherein the camera comprises a monochrome CCD camera.

5. The apparatus of claim 4, wherein the monochrome CCD camera has greater than about 350,000 pixels.

6. A method for making measurements of a blood cell population comprising the steps of:

providing a multiparameter hematology apparatus having a source of light energy configured to emit coherent light in a red spectrum, a transparent reservoir for containing a blood specimen diluted to less than 1/100,a camera for receiving an ensemble light scattering pattern from an ensemble of blood cells contained in the specimen contained in the reservoir and providing images thereof a digitizing means for digitizing images in operative communication with the camera for receiving and converting the images into digital images and a processor operatively connected to the digitizing the means for performing image analysis on the digital images;

providing a blood specimen diluted to less than 1/100 in the transparent reservoir of the multiparameter hematology apparatus;

irradiating the specimen with light from the source of light energy in the red spectrum;

picking up the ensemble light scattering pattern from the irradiated ensemble of blood cells contained in the specimen with the camera; and

converting the images of the ensemble light scattering pattern into digital images with the digitizing means ,and processing the digitized images of the ensemble light scattering pattern with the process to establish blood parameters for the blood specimen including intrinsic parameters such as cell size, extrinsic parameters such as cell counts as well as aggregation and sedimentation of blood cells.

7. The method as in claim 6, wherein the blood specimen is diluted with isotonic buffered saline including an anticoagulant and the established blood parameters are selected from the group consisting of red blood cell count and aggregation of blood cell.

8. The method as in claim 6, wherein the blood specimen is diluted with an isotonic reagent containing an active ingredient to sphere RBC (red blood cells) at constant volume and the established blood parameters are selected from the group consisting of MCV (mean cell volume), RBC, MCH (mean cell hemoglobin), MCHC (mean cell hemoglobin concentration), and PLT (platelets)/RBC.

9. The method as in claim 6, wherein the blood specimen is diluted with a hemolytic reagent configured to destroy RBC (red blood cells) while preserving WBC (white blood cells) and the established blood parameters are selected from the group consisting of WBC, GRAN (percentage of granulocytes), and AGRAN (percentage of agranulocytes).

10. The method as in claim 6, wherein the blood specimen is diluted with a hemolytic reagent capable of destroying RBC (red blood cells) and lysing WBC (white blood cells) and the established blood parameters are selected from the group consisting of WBC, PLT (platelets), POLY (polymorphonuclear cells) and MONO (mononuclear cells).

11. The method as in claim 6, wherein the step of picking up the ensemble light scattering pattern includes obtaining a measurement across a forward angle of between about 0°

and about 10°

relative to the blood specimen and the established blood parameters are selected from the group consisting of WBC (white blood cells), RBC (red blood cells), and PLT (platelets) concentration.

12. The method as in claim 6, wherein the of picking up the ensemble light scattering pattern includes picking up a multiple ring circularly symmetric scattering pattern wherein a diameter of a first ring corresponds to an average RBC (red blood cells) diameter.

13. The method as in claim 6, wherein the step of picking up the ensemble light scattering pattern includes taking intensity measurements at two angular intervals to generate first and second signals corresponding to the intensity of the ensemble scattered light within said angular intervals, and

establishing the blood parameters of MCV, (mean cell volume) and MCHC (mean cell hemoglobin concentration) by comparing said first and second signals to corresponding signals generated from blood populations of known MCV and MCHC.

14. The method as in claim 6, further comprising the steps of interposing a mask on the ensemble light scattering pattern, measuring a masked value of intensity, comparing a value of unmasked intensity with the masked value of intensity and establishing the blood parameters selected from the group consisting of PLT (platelets)/RBC (red blood cells), GRAN (percentage of granulocytes)/WBC (white blood cells), GRAN/AGRAN (percentage of agranulocytes), MONO(percentage mononuclear cells)/POLY (percentage of polymorphonuclear cells), and POLY/WBC.

15. The method as in claim 14, wherein the mask is physical and is interposed precedent to the camera.

16. The method as in claim 14, comprising interposing a virtual mask.

17. The method as in claim 6, wherein the step of processing the digitized images comprises determining ensemble light scattering intensity as a function of time and the established blood parameter is ESR (enthrocyte sedimentation rate).

18. The method as in claim 6, wherein the step of processing the digitized images comprises determining fluctuations in ensemble light scattering intensity as a function of time and the established blood parameter is ESR(enthroyte Sedimentation rate).