Fourier detection of species migrating in a microchannel
First Claim
1. A method of performing high-speed electrophoretic analysis of subject materials, said method comprising:
- holding said subject materials in solution in a channel of a microfluidic system;
subjecting said materials to an electric field so that said subject materials move through said channel and separate into species bands;
directing light toward said channel at periodically spaced regions along said channel simultaneously;
receiving light from said channel; and
analyzing frequencies of light intensity of said received light so that velocities of said bands along said channel can be determined for analysis of said materials.
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Abstract
The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.
369 Citations
26 Claims
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1. A method of performing high-speed electrophoretic analysis of subject materials, said method comprising:
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holding said subject materials in solution in a channel of a microfluidic system; subjecting said materials to an electric field so that said subject materials move through said channel and separate into species bands; directing light toward said channel at periodically spaced regions along said channel simultaneously; receiving light from said channel; and analyzing frequencies of light intensity of said received light so that velocities of said bands along said channel can be determined for analysis of said materials. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A detection apparatus for detecting separated species bands in a microscale channel, comprising:
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a light source arranged to direct light toward said channel only at periodically spaced regions along said channel; a photoreceptor arranged to receive light from said channel; and a processor unit connected to said photoreceptor to analyze frequencies of light intensity received by said photoreceptor so that velocities of said bands along said channel can be determined for analysis of said species bands. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
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Specification