Anti-cholesterolemic egg, vaccine and method for production, and use

• US 5,853,765 A
• Filed: 06/07/1995
• Issued: 12/29/1998
• Est. Priority Date: 06/03/1982
• Status: Expired due to Fees

• Current Assignee: Arkion Life Sciences
• Original Assignee: DCV, Inc. (Eastman Chemical Company)
• Inventors: Stolle, Ralph J., Beck, Lee R.
• Primary Examiner(s): Chambers, Jasemine C.
• Assistant Examiner(s): REYNOLDS, DEBORAH J
• Application Number:
  US08/487,419
• Time in Patent Office: 1,301 Days
• Field of Search: 426/614, 426/417, 426/480, 426/271, 426/425, 426/429, 426/478, 426/573, 426/575, 424/157.1, 424/203.1, 424/581, 424/439, 424/234.1, 424/282.1, 800/2, 119/6.8
• US Class Current: 424/581
• CPC Class Codes: A23L 15/00 Egg products; Preparation or treatment thereof
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 35/57 Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
• CPC Class Codes: C07K 16/02 from eggs
• CPC Class Codes: C07K 16/12 against material from bacteria
• CPC Class Codes: C07K 2317/11 isolated from eggs

Abstract

The present invention is directed to the treatment of vascular disorders particularly arteriosclerosis and atherosclerosis in warm-blooded animals. The invention encompasses the ingestion, by warm-blooded animals, of eggs or egg fractions derived from female avians that have been hyperimmunized with specific bacterial antigens or groups of bacterial antigens. The invention is directed to methods of controlling cholesterol levels, lipid deposits, and the development of atheromatous lesions in warm-blooded animals by the ingestion of eggs or fractions thereof produced in female avians hyperimmunized with specific bacterial antigens. The invention is also directed to methods of producing the eggs and to vaccines for hyperimmunization of the female avians.

Claims

1. A method for reducing the development of atheromatous lesions in a mammal being fed a high-cholesterol diet (HCD) to levels lower than those in said mammal being fed a HCD when untreated, said method comprising orally administering to said mammal egg or a fraction thereof, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one bacterial antigen from each of the following bacterial strains;

Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes A. Type 1, Streptococcus pyogenes A. Type 3, Streptococcus pyogenes A. Type 5, Streptococcus pyogenes A. Type 8, Streptococcus pyogenes A. Type 12, Streptococcus pyogenes A. Type 14, Streptococcus pyogenes A. Type 18, Streptococcus pyogenes A. Type 22, Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Streptococcus mitis, Proteus vulgaris, Shigella dysenteriae, Diplococcus pneumoniae, Propionibacter acnes (anaerobe), Streptococcus sanguis, Streptococcus salivarius, Streprococcus mutans, and Streptococcus agalactiae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition, and

collecting said egg from said female avian in said hyperimmune state.

2. The method of claim 1 wherein said egg fraction comprises egg white.

3. The method of claim 1 wherein said egg fraction comprises egg yolk IgY fraction.

18. The method of any one of claims 1, 4 or 7-17 wherein said warm-blooded animal is a human.

4. A method for lowering serum cholesterol in a mammal being fed a HCD to levels lower than those in said bring fed a HCD when untreated, said method comprising orally administering to said mammal egg or a fraction thereof, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one bacterial antigen derived from each of the following bacterial strains;

Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes A. Type 1, Streptococcus pyogenes A. Type 3, Streptococcus pyogenes A. Type 5, Streptococcus pyogenes A. Type 8, Streptococcus pyogenes A. Type 12, Streptococcus pyogenes A. Type 14, Streptococcus pyogenes A Type 18, Streptococcus pyogenes A. Type 22, Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Streptococcus mitis, Proteus vulgaris, Shigella dysenteriae, Diplococcus pneumoniae, Propionibacter acnes (anaerobe), Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Streptococcus agalactiae,

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

5. The method of claim 4 wherein said egg fraction comprises egg white.

6. The method of claim 4 wherein said egg fraction comprises egg yolk IgY fraction.

7. A method for lowering the level of lipid deposits in the aorta of a mammal, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with at least one antigen from Aerobacter aerogenes;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said bacterial antigen; and

collecting said egg from said female avian in said hyperimmune state.

8. A method for maintaining the level of lipid deposits in the aorta of a mammal comprising administering an egg or egg product to a mammal, wherein said egg or egg product does not alter the level of lipid deposits in the aorta of said mammal from those levels found in said mammal prior to administration, said method comprising administering to said mammal, said egg or egg product in an amount and for a time sufficient to produce said lipid-maintaining effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one antigen from each of the following bacterial strains;

Aerobacter aerogenes, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes A. Type 1, Streptococcus pyogenes A. Type 3, Streptococcus pyogenes A. Type 5, Streptococcus pyogenes A. Type 8, Streptococcus pyogenes A. Type 12, Streptococcus pyogenes A. Type 14, Streptococcus pyogenes A. Type 18, Streptococcus pyogenes A. Type 22, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Streptococcus mitis, Proteus vulgaris, Shigella dysenteriae, Diplococcus pneumoniae, Propionibacter acnes (anaerobe), Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Streptococcus agalactiae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

9. A method for maintaining the level of lipid deposits in the aorta of a mammal comprising administering an egg or egg product to a mammal wherein said egg or egg product does not alter the level of lipid deposits in the aorta of said mammal from the levels found in said mammal prior to administration, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid-maintaining effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one antigen from each of the following bacterial strains;

Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Proteus vulgaris, and Shigella dysenteriae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

10. A method for lowering the level of lipid deposits in the aorta of a mammal compared to the level of lipid deposits in the aorta of a mammal ingesting a normal egg comprising administering egg or egg product to a mammal wherein said egg or egg product does not elevate the level of lipid deposits in the aorta of said mammal to the level of lipid deposits found in the aorta of said mammal ingesting a normal egg, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with at least one antigen from at least one bacterial strain, said strain being selected from the group consisting of Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Shigella dysenteriae;

maintaining sad female avian in a hyperimmune state by administering to said female avian booster injections of said bacterial antigen; and

collecting said eggs from said female avian in said hyperimmune state.

11. A method for maintaining the level of lipid deposits in the liver of a mammal comprising administering an egg or egg product to a mammal wherein said egg or egg product does not alter the level of lipid deposits in the liver of said mammal from those levels found in said mammal prior to administration, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid-maintaining effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one bacterial antigen from each of the following bacterial strains;

Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes A. Type 1, Streptococcus pyogenes A. Type 3, Streptococcus pyogenes A. Type 5, Streptococcus pyogenes A. Type 8, Streptococcus pyogenes A. Type 12, Streptococcus pyogenes A. Type 14, Streptococcus pyogenes A. Type 18, Streptococcus pyogenes A. Type 22, Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Streptococcus mitis, Proteus vulgaris, Shigella dysenteriae, Diplococcus pneumoniae, Propionibacter acnes (anaerobe), Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Streptococcus agalactiae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

12. A method for maintaining the level of lipid deposits in the liver of a mammal comprising administering an egg or egg product to a mammal wherein said egg or egg product does not alter the level of lipid deposits in the liver of said mammal from those levels found in said mammal prior to administration, which comprises administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid-maintaining effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one bacterial antigen from each of the following bacterial strains;

Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Proteus vulgaris, Shigella dysenteriae

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

13. A method for maintaining the level of lipid deposits in the liver of a mammal comprising administering an egg or egg product to a mammal wherein said egg or egg product does not alter the level of lipid deposits in the liver of said mammal from those levels found in said mammal prior to administration, which comprises administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid-maintaining effect, wherein said egg is produced by a method comprising:

immunizing a female avian with at least one bacterial antigen from at least one bacterial strain, said bacterial strains selected from the group consisting of Aerobacter aerogenes and Escherichia coli;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said bacterial antigen; and

collecting said egg from said female avian in said hyperimmune state.

14. A method for lowering the level of lipid deposits in the liver of a mammal compared to the level of lipid deposits in the liver of a mammal ingesting a normal egg comprising administering egg or egg product to a mammal wherein said egg or egg product does not elevate the level of lipid deposits in the liver of said mammal to the level of lipid deposits found in the liver of said mammal ingesting a normal egg, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said lipid-lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with at least one antigen from at least one bacterial strain, said strain being selected from the group consisting of Salmonella enteritidis, Pseudomonas aeruginosa, and Klebsiella pneumoniae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said bacterial antigen; and

collecting said egg from said female avian in said hyperimmune state.

15. A method for lowering the serum cholesterol in a mammal compared to the level of serum cholesterol in a mammal ingesting a normal egg comprising administering egg or egg product to a mammal wherein said egg or egg product does not elevate the level of serum cholesterol in said mammal to the level found in said mammal ingesting a normal egg, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said serum cholesterol lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one antigen from each of the following bacterial strains;

Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, Proteus vulgaris, and Shigella dysenteriae;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

16. A method for lowering the serum cholesterol in a mammal compared to the level of serum cholesterol in a mammal ingesting a normal egg comprising administering egg or egg product to a mammal wherein said egg or egg product does not elevate the level of serum cholesterol in said mammal to levels found in said mammal ingesting a normal egg, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said serum cholesterol lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with a composition comprising at least one antigen from each of the following bacterial strains;

Streptococcus pyogenes A. Type 1, Streptococcus pyogenes A. Type 3, Streptococcus pyogenes A. Type 5, Streptococcus pyogenes A. Type 8, Streptococcus pyogenes A. Type 12, Streptococcus pyogenes A. Type 14, Streptococcus pyogenes A. Type 18, and Streptococcus pyogenes A. Type 22;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said composition; and

collecting said egg from said female avian in said hyperimmune state.

17. A method for lowering the serum cholesterol in a mammal compared to the level of serum cholesterol in a mammal ingesting a normal egg comprising administering egg or egg product to a mammal wherein said egg or egg product does not elevate the level of serum cholesterol in said mammal to levels found in said mammal ingesting a normal egg, said method comprising administering to said mammal, egg or egg product in an amount and for a time sufficient to produce said serum cholesterol lowering effect, wherein said egg is produced by a method comprising:

immunizing a female avian with at least one bacterial antigen from at least one bacterial strain, said bacterial strains selected from the group consisting of Aerobacter aerogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Haemophilus influenzae, and Proteus vulgaris;

maintaining said female avian in a hyperimmune state by administering to said female avian booster injections of said bacterial antigen; and

collecting said egg from said female avian in said hyperimmune state.