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Use of nucleic acid ligands in flow cytometry

  • US 5,853,984 A
  • Filed: 06/07/1995
  • Issued: 12/29/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence of a target compound in a substance which may contain said target compound, wherein said target compound or said substance is particulate, comprising:

  • a) identifying a nucleic acid ligand from a candidate mixture of nucleic acids, said nucleic acid ligand being a ligand of said target compound, by the method comprising;

    i) contacting the candidate mixture with said target compound, wherein nucleic acids having an increased affinity to said target compound relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;

    ii) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;

    iii) amplifying the increased affinity nucleic acids to yield a ligand-enriched mixture of nucleic acids; and

    iv) identifying said nucleic acid ligand;

    b) attaching said nucleic acid ligand with a fluorophore to form a fluorophore-labelled nucleic acid ligand;

    c) mixing said fluorophore-labelled nucleic acid ligand with a substance which may contain said target compound under conditions suitable for binding of the fluorophore-labelled nucleic acid ligand to said target compound; and

    d) analyzing said mixture by flow cytometry to detect whether said fluorophore-labelled nucleic acid ligand bound to said target compound in said substance, whereby the presence of a target compound in a substance can be detected.

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