Polymerized crystalline colloidal array sensor methods

• US 5,854,078 A
• Filed: 03/17/1997
• Issued: 12/29/1998
• Est. Priority Date: 11/06/1996
• Status: Expired due to Term

• Current Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Original Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Inventors: Asher, Sanford A., Holtz, John H.
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US08/819,240
• Time in Patent Office: 652 Days
• Field of Search: 436/133-136, 436/138, 436/164, 436/167, 436/169, 436/183, 436/535, 436/73, 436/79, 436/95, 435/14
• US Class Current: 436/133
• CPC Class Codes: B01J 13/00 Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
• CPC Class Codes: B01J 13/0065 containing an organic phase
• CPC Class Codes: G01J 3/18 using diffraction elements, e.g. grating gratings per se G02B
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/7723 Swelling part, also for adsorption sensor, i.e. without chemical reaction
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 31/222 for investigating moisture content
• CPC Class Codes: G02B 26/002 the movement or the deformation controlling the frequency of light, e.g. by Doppler effect
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/204998 Inorganic carbon compounds
• CPC Class Codes: Y10T 436/205831 Carbon monoxide only
• CPC Class Codes: Y10T 436/207497 Molecular oxygen
• CPC Class Codes: Y10T 436/209163 Dissolved or trace oxygen or oxygen content of a sealed environment

Abstract

Novel sensor devices composed of a crystalline colloidal array (CCA) polymerized in a hydrogel are disclosed. The hydrogels are characterized as being capable of shrinking and swelling in response to specific stimuli applied thereto. As the hydrogels shrink or swell, the lattice structure of the CCA embedded therein changes, thereby changing the wavelength of light diffracted by the CCA. Thus by monitoring the change in diffracted wavelength, the concentration of a stimulus is determined. The gels can be modified to sense numerous different stimuli. The sensor devices are specific in that they are modified to react with only one species or family of species. These sensors have various applications in areas including, for example, environmental and chemical systems, chemomechanical systems, sensor devices and medical diagnostic tools. Various methods for making and using these devices are also disclosed.

Claims

1. A method for detecting the concentration of a gas in a solution comprising:

preparing a sensor device comprising a crystalline colloidal array polymerized in a hydrogel that undergoes a volume change in response to said gas, said crystalline colloidal array having a lattice spacing that changes when said volume of said hydrogel changes, thereby causing the diffracted wavelength of the crystalline colloidal array to change;

measuring the diffracted wavelength of said crystalline colloidal array polymerized in said hydrogel;

contacting said polymerized crystalline colloidal array with said solution;

measuring the diffracted wavelength of said crystalline colloidal array following exposure to said solution; and

comparing the change in diffracted wavelength measurements to determine the concentration of said gas.

2. The method of claim 1, wherein the presence of said chemical species is determined by inspection of the diffracted wavelength by the polymerized crystalline colloidal array both before and after exposure to said solution, wherein a change in the wavelength diffracted by said array indicates the presence of the chemical species.

3. The method of claim 2, including employing the use of a detection device selected from the group consisting of a spectrometer or a spectrophotometer to determine said diffracted wavelength change.

4. The method of claim 2, including employing the unassisted human eye to determine said diffracted wavelength change, wherein such a change is detectable by a change in color of the sensor device.

5. The method of claim 1, wherein said sensor device preparation step includes the steps of:

a) allowing charged colloidal particles to self-assemble into a crystalline colloidal array;

b) adding a first comonomer that is a gel monomer, a crosslinking agent, a second comonomer capable of molecular recognition and a polymerization initiator to a medium comprising said crystalline colloidal array; and

c) polymerizing the mixture of step b) to form a crystalline colloidal array embedded in a hydrogel.

6. The method of claim 5, including employing a medium that is hydrophilic.

7. The method of claim 5, including employing a gel monomer that is an ion-free gel.

8. The method of claim 5, including employing a gel monomer selected from the group consisting of acrylamide gels, purified agarose gels, N-vinylpyrolidone gels, and methacrylate gels.

9. The method of claim 8, including employing as said gel monomer N-isopropylacrylamide.

10. The method of claim 5, including employing a molecular recognition monomer selected from the group consisting of crown ethers, cyclodextrans and caloxarenes.

11. The method of claim 10, including employing a 4-acrylamidobenzo 18-crown-6 ether.

12. The method of claim 5, including employing a crosslinking agent selected from the group consisting of N,N'"'"'-methylenebisacrylamide, methylenebismethacrylamide and ethyleneglycol-dimethacrylate.

13. The method of claim 12, including employing as said crosslinking agent N, N'"'"'-methylenebisacrylamide.

14. The method of claim 5, including employing charged particles selected from the group consisting of colloidal polystyrene, polymethylmethacrylate, silicon dioxide, aluminum oxide, polytetrafluoroethylene and poly N-isopropylacrylamide.

15. The method of claim 5, including employing in said hydrogel a third monomer.

16. The method of claim 15, including employing as said third monomer an acrylamide or a substituted acrylamide.

17. The method of claim 1, wherein said sensor device preparation step further comprises:

a) allowing charged colloidal particles to self assemble into a crystalline colloidal array;

b) adding a gel monomer, a crosslinking agent and a polymerization initiator to a medium comprising said crystalline colloidal array;

c) polymerizing the mixture of step b) to form a crystalline colloidal array embedded in a hydrogel; and

d) adding a biomolecular recognition component to the product of step c).

18. The method of claim 17, wherein said biomolecular recognition component is added to the product of step c) by use of one or more linking molecules.

19. The method of claim 18, wherein said biomolecular recognition component is reacted with a linking molecule that can be bound to a second linking molecule or to the gel.

20. The method of claim 19, including employing avidin as the linking molecule that can be bound to the second linking molecule or to the gel.

21. The method of claim 18, including employing as said linking molecule 5-(biotinamido)pentylamine.

22. The method of claim 17, including the step of hydrolyzing the polymerized crystalline colloidal array of step c) before adding a biomolecular recognition component.

23. The method of claim 17, including employing said sensor device to detect a particular chemical species by using a biomolecular recognition component capable of recognizing the stimulus to be detected.

24. The method of claim 17, including employing a UV photoinitiator and wherein said polymerization step is effected by exposing the mixture of step b) to UV light.

25. The method of claim 17, including employing a gel monomer selected from the group consisting of acrylamide gels, purified agarose gels, N-vinylpyrolidone gels, and methacrylate gels.

26. The method of claim 25, including employing as said gel monomer N-isopropylacrylamide.

27. The method of claim 17, including employing a biomolecular recognition component selected from the group consisting of enzymes, antigens, nucleic acids, nucleic acid sequences, amino acids, amino acid sequences, peptides and antibodies.

28. The method of claim 27, wherein said biomolecular recognition component is glucose oxidase and said gas is dissolved oxygen.

29. The method of claim 17, including employing a crosslinking agent selected from the group consisting of N,N'"'"'-methylenebisacrylamide, methylenebismethacrylamide and ethyleneglycol-dimethacrylate.

30. The method of claim 29, including employing as said crosslinking agent N,N'"'"'-methylenebisacrylamide.

31. The method of claim 17, including employing charged particles selected from the group consisting of colloidal polystyrene, polymethylmethacrylate, silicon dioxide, aluminum oxide, polytetrafluoroethylene and poly N-isopropylacrylamide.

32. The method of claim 1, wherein said gas is selected from the group consisting of oxygen, carbon monoxide, and carbon dioxide.

33. The method of claim 1, wherein said gas is dissolved oxygen.