ETS2 repressor factor (ERF) genetic locus and its products

• US 5,856,125 A
• Filed: 06/05/1995
• Issued: 01/05/1999
• Est. Priority Date: 06/05/1995
• Status: Expired due to Term

• Current Assignee: Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services
• Original Assignee: United States Department Of Health And Human Services (Government of the United States of America)
• Inventors: Fisher, Robert J., Blair, Donald G., Sgouras, Dionyssios N., Mavrothalassitis, George J., Athanasiou, Meropi A., Beal, Gregory J. Jr.
• Primary Examiner(s): Walsh, Stephen
• Assistant Examiner(s): Pak, Michael D.
• Application Number:
  US08/469,412
• Time in Patent Office: 1,310 Days
• Field of Search: 536/23.5, 536/23.4, 536/24.31, 530/350, 530/387.1, 514/2, 514/44, 435/240.2, 435/325
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 14/4703 Inhibitors; Suppressors
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

The present invention relates, inter alia, to the ERF gene and to the products encoded by this gene. More particularly, the present invention relates to DNA sequences encoding ERF and AERF; polypeptides encoded by such DNA sequences; ERF chimeric molecules; and methods of using ERF and ERF chimeric molecules to reduce tumorigenicity in a tumor cell.

Claims

1. An isolated DNA sequence which encodes an ETS2 Repressor Factor protein and said DNA sequence hybridizes under high stringency conditions to an ETS2 Repressor Factor gene in a human genomic library, said ETS2 Repressor Factor gene having a nucleic acid sequence comprising SEQ ID NO:

1.

2. The isolated DNA sequence in accordance with claim 1, wherein said DNA sequence encodes an ETS2 Repressor Factor protein having an amino acid sequence comprising SEQ ID NO:

2.

4. The isolated DNA sequence in accordance with claim 1 wherein said isolated DNA sequence hybridizes under high stringency conditions to an ETS2 Repressor Factor gene in human genomic library, said ETS2 Repressor Factor gene having a nucleic acid sequence comprising SEQ ID NO:

1.

3. An isolated DNA sequence which encodes an ETS2 Repressor Factor (ERF) protein, said ETS2 Repressor Factor protein having an amino acid sequence comprising SEQ ID NO:

2.

5. The isolated DNA sequence in accordance with claim 3 wherein said isolated DNA sequence has a nucleic acid sequence comprising SEQ ID NO:

1.

6. An isolated DNA sequence which encodes an ETS2 Repressor factor protein, said ETS2 Repressor Factor protein specifically binding to a polyclonal antibody generated against an immunogen having an amino acid sequence comprising SEQ ID NO:

2.

7. An isolated DNA sequence which encodes an ETS2 Repressor Factor protein and said DNA sequence hybridizes under high stringency conditions to an ETS2 Repressor Factor gene in a human genomic library, said ETS2 Repressor Factor gene having a nucleic acid sequence comprising SEQ ID NO:

3.

8. An isolated DNA sequence, wherein said DNA sequence encodes a human ETS2 Repressor Factor protein having an amino acid sequence comprising SEQ ID NO:

4.

9. An isolated DNA sequence which encodes a human ETS2 Repressor Factor protein, said human ETS2 Repressor Factor protein having an amino acid sequence comprising SEQ ID NO:

4.

10. The isolated DNA sequence in accordance with claim 9 wherein said isolated DNA sequence hybridizes under high stringency conditions to an ETS2 Repressor Factor gene in a human genomic library, said ETS2 Repressor Factor gene having a nucleic acid sequence comprising SEQ ID NO:

3.

11. The isolated DNA sequence in accordance with claim 9 wherein said isolated DNA sequence has a nucleic acid sequence comprising SEQ ID NO:

3.

12. An isolated DNA sequence which encodes an ERF protein, said ETS2 Repressor Factor protein specifically binding to a polyclonal antibody generated against an immunogen having an amino acid sequence comprising SEQ ID NO:

4.