Human stem cell antigens

• US 5,856,136 A
• Filed: 07/03/1996
• Issued: 01/05/1999
• Est. Priority Date: 07/03/1996
• Status: Expired due to Fees

• Current Assignee: Incyte Pharmaceuticals, Inc.
• Original Assignee: Incyte Pharmaceuticals, Inc.
• Inventors: Au-Young, Janice
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): Nelson, Amy J.
• Application Number:
  US08/675,508
• Time in Patent Office: 916 Days
• Field of Search: 536/23.5, 435/320.1, 435/240.2, 435/69.3, 435/243, 435/252.3, 435/325
• US Class Current: 435/69.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

The present invention provides polynucleotides encoding stem cell antigens, the partial sequences for which were initially isolated from THP-1 and bladder tumor cDNA libraries and which identify and encode novel human stem cell antigens. The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding the stem cell antigens.

Claims

1. A purified polynucleotide encoding a polypeptide with an amino acid sequence shown in SEQ ID NO:

2.

3. An expression vector comprising the polynucleotide of claim 1.

4. A host cell transformed with the expression vector of claim 3.

5. A method for producing a polypeptide with the amino acid sequence of SEQ ID NO:

2, the method comprising the steps of;

a) culturing the host cell of claim 4 to allow expression of the polypeptide; and

b) recovering the polypeptide from the host cell culture.

2. The polynucleotide consisting of the nucleic acid sequence of SEQ ID NO:

4, or its complement.