Acrylic microchannels and their use in electrophoretic applications
First Claim
1. In a method where charged entities are moved through a channel under the influence of an applied voltage differential, the improvement comprising the step of employing a microchannel formed from a surface of a polymethylmethacrylate material for reducing at least one of electroosmotic flow or adsorption as compared to a microchannel formed from native fused silica.
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Accused Products
Abstract
Microchannels having at least an acrylic inner surface and methods of their use in electrophoretic applications are provided. The subject microchannels may be in the form of a variety of configurations suitable for holding an electrophoretic medium. The subject microchannels give rise to substantially reduced EOF and/or adsorption as compared to fused silica under conditions of electrophoresis and find use in a variety of electrophoretic applications in which charged entities are moved through a medium under the influence of the an applied electric field.
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Citations
45 Claims
- 1. In a method where charged entities are moved through a channel under the influence of an applied voltage differential, the improvement comprising the step of employing a microchannel formed from a surface of a polymethylmethacrylate material for reducing at least one of electroosmotic flow or adsorption as compared to a microchannel formed from native fused silica.
- 4. In a method where charged entities are moved through a channel under the influence of an applied voltage differential, the improvement comprising the step of employing a microchannel formed from a surface of an acrylic copolymer material for reducing at least one of electroosmotic flow or adsorption as compared to a microchannel formed from native fused silica.
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11. In a method of electrophoretic separation of a sample in which sample components are separated in an electrophoretic separation medium in a channel by application of a voltage differential to said medium, the improvement comprising the steps of employing a microchannel formed from a surface of an acrylic material and having cross-sectional inner dimensions ranging from about 1 to 200 μ
- m, the acrylic material being optically transparent, and optically detecting separated sample components in said microchannel.
- View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
- 19. In a method of electrophoretic separation of a sample in which sample components are separated in an electrophoretic separation medium in a channel by application of a voltage differential to said medium, the improvement comprising the step of employing a microchannel formed from a groove in a surface of a polymethylmethacrylate substrate and having cross-sectional inner dimensions ranging from about 1 to 200 μ
- 22. In a method of electrophoretic separation of a sample in which sample components are separated in an electrophoretic separation medium in a channel by application of a voltage differential to said medium, the improvement comprising the step of employing a polymethylmethacrylate capillary having an inner diameter ranging from about 10 to 200 μ
- 25. A device for use in a method were charged entities are moved through a channel under the influence of an applied voltage differential comprising a body at least partially made from an acrylic material, said body having a surface and a microchannel provided in said surface adapted to receive said charged entities for movement under the influence of said applied voltage differential, said microchannel formed by the acrylic material and having cross-sectional inner dimensions ranging from about 1 to 200 μ
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31. In an electrophoretic device for use in electrophoretic applications in which charged entities are moved through an electrophoretic medium in response to an applied voltage differential, said device having a microchannel and an electrophoretic medium disposed in said microchannel, the improvement comprising said device being provided with a wall of an acrylic material for forming said microchannel, said microchannel having cross-sectional inner dimensions ranging from about 1 to 200 μ
- m, said wall of an acrylic material being optically transparent to permit optical detection of said charged entities within said microchannel.
- View Dependent Claims (32, 33, 34, 35, 38)
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36. A kit for use in a method where charged entities are moved through a channel under the influence of an applied voltage differential comprising a component provided with a microchannel formed from an acrylic copolymer, said microchannel having cross-sectional inner dimensions ranging from about 1 to 200 μ
- m, and an electrophoretic medium for disposition in said microchannel.
- View Dependent Claims (37)
- 39. A device for use in electrophoretic applications in which charged entities are moved through an electrophoretic medium for separation in response to a voltage differential applied across the electrophoretic medium and the charged entities disposed therein comprising a body provided with a microchannel adapted for receiving said electrophoretic medium and said charged entities for electrophoretic separation, said body having a portion made from polymethylmethacrylate for forming said microchannel, said microchannel having cross-sectional inner dimensions ranging from 1 to 200 μ
- 44. A device for use in electrophoretic applications in which charged entities are moved through an electrophoretic medium for separation in response to a voltage differential applied across the electrophoretic medium and the charged entities disposed therein comprising a body provided with a microchannel adapted for receiving said electrophoretic medium and said charged entities for electrophoretic separation, said body having a portion made from an acrylic copolymer for forming a microchannel, said microchannel having cross-sectional inner dimensions ranging from 1 to 200 μ
Specification