Method and apparatus for manipulation using spiral electrodes

• US 5,858,192 A
• Filed: 10/18/1996
• Issued: 01/12/1999
• Est. Priority Date: 10/18/1996
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Huang, Ying, Becker, Frederick F., Gascoyne, Peter R. C., Wang, Xiao-Bo
• Primary Examiner(s): Warden, Robert
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/733,508
• Time in Patent Office: 816 Days
• Field of Search: 204/547, 204/670, 7/400, 7/280, 7/643, 436/149, 436/806
• US Class Current: 204/547
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00853 Employing electrode arrangements
• CPC Class Codes: B01J 2219/00905 Separation
• CPC Class Codes: B01J 2219/00914 by dielectrophoresis
• CPC Class Codes: B03C 5/028 using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
• CPC Class Codes: G01N 2030/0035 electrical field
• CPC Class Codes: G01N 30/0005 Field flow fractionation
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of a suspending medium to discriminate and separate such matter. The apparatus includes a chamber having at least one spiral electrode element. Matter is separated in the chamber by a dielectrophoretic (DEP) force caused by the energized electrode or electrodes.

Claims

1. An apparatus for the discrimination of matter utilizing dielectrophoresis comprising:

a chamber having an interior surface and an exterior surface;

at least one spiral electrode element positioned along a portion of said chamber; and

an electrical signal generator to energize said at least one spiral electrode element and create an electric field that causes a dielectrophoretic force on said matter within said chamber, thereby displacing said matter to positions within said chamber.

2. The apparatus according to claim 1, further comprising one inlet port and one outlet port within said chamber.

3. The apparatus according to claim 1, further comprising a plurality of spiral electrode elements arranged in a spiral electrode array.

4. The apparatus according to claim 3, wherein each of said plurality of spiral electrode elements is individually connected to one of a plurality of electrical conductors electrically connected to an electrical signal generator.

5. The apparatus according to claim 3, wherein each of said plurality of spiral electrode elements is energized at different phases to create an energized spiral electrode array.

6. The apparatus according to claim 5, wherein said energized spiral electrode array further creates a traveling electric field.

7. The apparatus according to claim 3, wherein the spiral electrode array is configured on a top wall of said chamber.

8. The apparatus according to claim 3, wherein the spiral electrode array is configured on a bottom wall of said chamber.

9. The apparatus according to claim 3, wherein a plurality of said spiral electrode arrays are adapted on opposing surfaces of said chamber.

10. The apparatus according to claim 1, wherein said dielectrophoresis comprises conventional dielectrophoresis.

11. The apparatus according to claim 1, wherein said dielectrophoresis comprises traveling wave dielectrophoresis.

12. The apparatus according to claim 1, wherein said dielectrophoresis comprises generalized dielectrophoresis.

13. The apparatus according to claim 1, wherein the chamber includes a top wall, a bottom wall, and a peripheral wall.

14. The apparatus according to claim 1, wherein said matter comprises particulate matter.

15. The apparatus according to claim 14, wherein the particulate matter comprises a cell, cell aggregate, cell organelle, nucleic acid, bacteria, protozoan, or virus.

16. The apparatus according to claim 1, wherein said matter comprises solubilized matter.

17. The apparatus according to claim 16, wherein the solubilized matter comprises a molecule, molecular aggregate, or molecule mixture.

18. The apparatus according to claim 17, wherein the molecule or molecular aggregate comprises a protein or a protein mixture.

19. The apparatus according to claim 1, wherein the at least one spiral electrode element is positioned along the interior surface of said chamber.

20. The apparatus according to claim 1, wherein the at least one spiral electrode element is adapted along the exterior surface of said chamber.

21. The apparatus according to claim 1, wherein said at least one electrical signal is provided by an electrical signal generator.

22. The apparatus according to claim 1, in which said at least one electrical signal comprises at least three electrical signals provided at different phases.

23. A method of discriminating matter utilizing dielectrophoresis, comprising:

providing a chamber, said chamber having an interior surface and an exterior surface and at least one spiral electrode element positioned along a portion of said chamber;

introducing said matter into said chamber;

applying at least one electrical signal to said at least one spiral electrode element to create an energized at least one spiral electrode element to cause a dielectrophoretic force on said matter; and

wherein said matter is displaced to positions within said chamber, thereby discriminating said matter according to its positions within said chamber.

24. The method according to claim 23, wherein said at least one electrical signal is provided by an electrical signal generator.

25. The method according to claim 23, wherein said energized at least one spiral electrode element creates a spatially inhomogeneous electric field.

26. A method according to claim 25, wherein said spatially inhomogeneous electric field causes said matter to be attracted to said at least one spiral electrode element.

27. A method according to claim 26, further comprising holding said matter in close proximity to said at least one spiral electrode element.

28. A method according to claim 26, wherein said spatially inhomogeneous electric field causes said matter to be repelled from said at least one spiral electrode element.

29. The method according to claim 23, further comprising at least three electrical signals provided at different phases to create a traveling electric field.

30. The method according to claim 23, wherein the matter is particulate matter.

31. The method according to claim 30, wherein the particulate matter comprises a cell, cell aggregate, cell organelle, nucleic acid, bacteria, protozoan, or virus.

32. The method according to claim 30, wherein the particulate matter comprises a mixture of cell types.

33. The method according to claim 32, wherein the mixture of cell types include cancer cells to be discriminated.

34. The method according to claim 32, wherein the mixture of cell types are a mixture of blood cells and breast cancer cells, said breast cancer cells to be discriminated.

35. The method according to claim 23, wherein said matter is solubilized matter.

36. The method according to claim 35, wherein the solubilized matter comprises a molecule, or molecular aggregate.

37. The method according to claim 36, wherein the molecule or molecular aggregate is a protein.

38. The method according to claim 23, further comprising varying said at least one electrical signal, thereby causing changes in displacement of said matter, thereby further discriminating said matter according to its positions within said chamber.

39. A method according to claim 23, wherein said matter includes desired matter and nondesired matter.

40. A method according to claim 39, in which said nondesired matter is held in close proximity to said at least one spiral electrode element and said desired matter is partitioned by said dielectrophoretic force.

41. A method according to claim 40, further comprising changing said at least one electrical signal, thereby releasing said nondesired matter from close proximity to said at least one spiral electrode element and partitioning said nondesired matter by said dielectrophoretic force.

42. A method according to claim 39, in which said desired matter is held in close proximity to said at least one spiral electrode element and said nondesired matter is partitioned by said dielectrophoretic force.

43. A method according to claim 42, further comprising changing said at least one electrical signal, thereby releasing said desired matter from close proximity to said at least one said plurality of electrode element and partitioning said desired matter by said dielectrophoretic force.

44. A method of determining characteristics of an unknown matter utilizing dielectrophoresis, comprising:

providing a chamber having an interior surface and an exterior surface;

a spiral electrode array positioned along a portion of said chamber;

introducing a mixture including said unknown matter into said chamber;

applying at least one electrical signal having an initial frequency to said spiral electrode array at different phases to create an energized spiral electrode array to cause a dielectrophoretic force on said unknown matter; and

varying said initial frequency until said unknown matter is caused to be moved to a central portion of said spiral electrode array at a discriminating frequency.

45. The method of claim 44, further comprising collecting said unknown matter at an outlet port of said chamber.

46. The method of claim 44, further comprising characterizing said unknown matter to identify said unknown matter.

47. A method for diagnosing a condition by determining a presence of unidentified matter in a patient sample utilizing dielectrophoresis, comprising:

obtaining a chamber having an interior surface and an exterior surface;

a spiral electrode array positioned along a portion of said chamber;

introducing a mixture including said unidentified matter into said chamber;

applying at least one electrical signal to said spiral electrode array at different phases at a discriminating frequency of a known matter to create a traveling electric field to cause a dielectrophoretic force on said mixture; and

determining whether said unidentified matter is moved to a central portion of said spiral electrode array, indicating said condition.

48. An apparatus for the discrimination of matter, comprising:

a chamber having an interior surface and an exterior surface, a top wall, a bottom wall and a peripheral wall;

a spiral electrode array positioned along a portion of at least one of said walls;

an electrical signal generator electrically connected to said spiral electrode array; and

wherein said spiral electrode array energized at different phases by at least one electrical signal provided by said electrical signal generator creates an electric field in said chamber, thereby causing at least one dielectrophoretic force on said matter.