Microplates with UV permeable bottom wells
First Claim
1. A microplate for use in assaying samples, comprising:
- a frame that forms sidewalls of at least one well; and
a first layer that forms a bottom of the at least one well, the first layer being formed from a plastic material having an average optical density that is no more than approximately 0.09 at a thickness of approximately 7.5 mils between wavelengths of approximately 200 nm and approximately 300 nm;
the bottom of the at least one wall having a thickness of less than approximately 14 mils.
3 Assignments
0 Petitions
Accused Products
Abstract
Microplates and methods for manufacturing microplates. The microplate is designed to allow UV radiation to pass through the bottom wells of the microplate so that the microplate can be used for assaying samples by use of UV absorbance. In one embodiment, the microplate comprises at least first and second wells, each well having a UV permeable bottom. In another embodiment, the microplate comprises a frame having an upper portion and a lower portion contiguous with the upper portion and a sheet disposed between the upper portion and the lower portion and defining the bottom of at least one well of the microplate. One embodiment of the method includes steps of inserting a sheet of UV permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten plastic material into the mold cavity, and cooling the plastic material to form the microplate with the plastic material forming the sidewalls of each of the first and second wells and the sheet of UV permeable material forming the bottom of each of the first and second wells. Another embodiment of the method includes providing an upper plate defining sidewalls of at least one well, adhering an intermediate layer to the upper plate and adhering a sheet of UV permeable material to the intermediate layer. A further embodiment of the method includes inserting a sheet of material having at least one hole into a mold cavity, injecting a molten plastic material into the mold cavity and cooling the plastic material to form a microplate.
84 Citations
24 Claims
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1. A microplate for use in assaying samples, comprising:
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a frame that forms sidewalls of at least one well; and a first layer that forms a bottom of the at least one well, the first layer being formed from a plastic material having an average optical density that is no more than approximately 0.09 at a thickness of approximately 7.5 mils between wavelengths of approximately 200 nm and approximately 300 nm; the bottom of the at least one wall having a thickness of less than approximately 14 mils. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A microplate for use in assaying samples, comprising:
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a frame including an upper portion and a lower portion, the upper portion defining sidewalls of at least one well, the upper portion and the lower portion being contiguous; and a sheet defining a bottom of the at least one well, at least a portion of the sheet being disposed between the upper and lower portions of the frame; the sheet having an average optical density that is no more than approximately 0.09 at a thickness of approximately 7.5 mils between wavelengths of approximately 200 nm and approximately 300 nm. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification