Method for diagnosing neoplasia by detecting expression of PRAD1 cyclin

• US 5,858,655 A
• Filed: 06/02/1995
• Issued: 01/12/1999
• Est. Priority Date: 03/11/1991
• Status: Expired due to Term

• Current Assignee: Diffracto, Ltd. (LMI Technologies Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Arnold, Andrew
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): ALLEN, MARIANNE P
• Application Number:
  US08/460,694
• Time in Patent Office: 1,320 Days
• Field of Search: 435/6, 536/23.1, 935/77, 935/78
• US Class Current: 435/6.14
• CPC Class Codes: A01K 2207/15 Humanized animals
• CPC Class Codes: A01K 2217/00 Genetically modified animals
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/0331 Animal model for proliferative diseases
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4738 Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR cell cycle dependent kinases C12N9/12
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic

Abstract

A novel cyclin, termed prad1, is disclosed, along with the nucleic acid sequence that encodes human prad1. Antibodies that bind specifically to prad1 and cell lines and transgenic animals containing DNA that encodes prad1 are also disclosed. In addition, methods of using the antibodies or fragments of the nucleic acid sequence, for example, to diagnose a neoplastic condition by detecting expression of prad1 or to modulate cellular proliferation, are disclosed.

Claims

1. A method for diagnosing neoplasia by detecting expression level of PRAD1 cyclin comprising:

obtaining a nucleic acid sample from an animal suspected of having a neoplastic condition;

contacting said nucleic acid sample with a single-stranded DNA comprising a segment of a PRAD 1 at least 8 nucleotides in length, said Prad1being the Prad1homolog of the species to which said animal belongs; and

detecting the level of hybridization of said single-stranded DNA with said nucleic acid sample, said level being diagnostic for said neoplastic condition.

2. The method of claim 1, wherein said animal is a mammal.

3. The method of claim 2, wherein said mammal is a human.

4. The method of claim 1, wherein said neoplastic condition is breast cancer.

5. The method of claim 1, wherein said neoplastic condition is a lymphoma.

6. The method of claim 1, wherein said neoplastic condition is a squamous cell cancer.

7. The method of claim 1, wherein said neoplastic condition is parathyroid adenoma.