Human retinol dehydrogenase type II homolog

• US 5,858,750 A
• Filed: 11/25/1997
• Issued: 01/12/1999
• Est. Priority Date: 11/25/1997
• Status: Expired due to Fees

• Current Assignee: Incyte Pharmaceuticals, Inc. (Incyte Corporation)
• Original Assignee: Incyte Pharmaceuticals, Inc. (Incyte Corporation)
• Inventors: Corley, Neil C., Lal, Preeti, Bandman, Olga
• Primary Examiner(s): Hendricks, Keith D.
• Assistant Examiner(s): Mayhew, Bradley S.
• Application Number:
  US08/977,847
• Time in Patent Office: 413 Days
• Field of Search: 435/190, 435/252.3, 435/252.33, 435/325, 435/419, 435/320.1, 536/23.2, 536/24.31
• US Class Current: 435/190
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)

Abstract

The invention provides a human retinol dehydrogenase type II homolog (HRoDH) and polynucleotides which identify and encode HRoDH. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of HRoDH.

Claims

1. An isolated and purified polynucleotide sequence encoding the amino acid sequence of SEQ ID No:

1.

2. A composition comprising the polynucleotide sequence of claim 1.

3. An isolated and purified polynucleotide sequence which is completely complementary to the polynucleotide sequence of claim 1.

6. An expression vector containing the polynucleotide sequence of claim 1.

7. A host cell containing the expression vector of claim 6.

8. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

1, the method comprising the steps of;

(a) culturing the host cell of claim 7 under conditions suitable for the expression of the polypeptide; and

(b) recovering the polypeptide from the host cell culture.

9. A method for detecting a polynucleotide encoding HRoDH in a biological sample containing nucleic acids, the method comprising the steps of:

(a) hybridizing the polynucleotide of claim 3 to at least one of the nucleic acids of the biological sample, thereby forming a hybridization complex; and

(b) detecting the hybridization complex, wherein the presence of the hybridization complex correlates with the presence of a polynucleotide encoding HRoDH in the biological sample.

10. The method of claim 9 wherein the nucleic acids of the biological sample are amplified by the polymerase chain reaction prior to the hybridizing step.

4. An isolated and purified polynucleotide sequence comprising SEQ ID No:

2.

5. An isolated and purified polynucleotide sequence which is completely complementary to the polynucleotide sequence of claim 4.