Immunological assay conducted in a microlaboratory array
First Claim
1. A method of performing an immunological assay separately and in parallel for each of at least a first sample containing or prospectively containing a first binding moiety and a second sample containing or prospectively containing the first binding moiety, the method comprising:
- placing the first sample and the second sample into an array comprising a solid substrate having an upper surface and a lower surface, a plurality of reservoirs, a plurality of wells connected to the reservoirs and channels that provide the connections between the wells and the reservoirs, wherein at least one channel is a crossover channel formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, thereby forming crossovers comprising crossing but non-intersecting channels;
moving said first sample into a first well containing a second binding moiety that binds to the first binding moiety, wherein the second binding moiety is bound to within the first well;
moving said second sample into a second well within which the second binding moiety is bound;
parallelly pumping from the reservoirs one or more fluids into the first and second wells to develop a detectable result that varies depending on the amount of the first binding moiety present in the first or second wells; and
measuring the detectable result.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides a method of performing an immunological assay separately and in parallel for each of at least a first sample containing or prospectively containing a first binding moiety, which can be an antigen or an antibody, and a second sample containing or prospectively containing the first binding moiety, the method comprising: placing the first sample and the second sample into an array comprising a solid substrate, a plurality of wells and channels, wherein at least one channel is a crossover channel formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, thereby forming crossovers comprising crossing but non-intersecting channels; moving said first sample into a first well containing a second binding moiety, which can be an antigen or an antibody, that binds to the first binding moiety, wherein the second binding moiety is bound to the walls of the first well; moving said second sample into a second well containing the second binding moiety bound to the walls thereof; and parallelly pumping one or more fluids through the first and second wells to develop a detectable result that varies depending on the amount of the first binding moiety present in the first or second wells.
-
Citations
18 Claims
-
1. A method of performing an immunological assay separately and in parallel for each of at least a first sample containing or prospectively containing a first binding moiety and a second sample containing or prospectively containing the first binding moiety, the method comprising:
-
placing the first sample and the second sample into an array comprising a solid substrate having an upper surface and a lower surface, a plurality of reservoirs, a plurality of wells connected to the reservoirs and channels that provide the connections between the wells and the reservoirs, wherein at least one channel is a crossover channel formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, thereby forming crossovers comprising crossing but non-intersecting channels; moving said first sample into a first well containing a second binding moiety that binds to the first binding moiety, wherein the second binding moiety is bound to within the first well; moving said second sample into a second well within which the second binding moiety is bound; parallelly pumping from the reservoirs one or more fluids into the first and second wells to develop a detectable result that varies depending on the amount of the first binding moiety present in the first or second wells; and measuring the detectable result. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A method of performing an immunological assay which comprises:
-
a) providing substrate having formed therein (i) at least a first reservoir and a second reservoir, (ii) a plurality of wells that each have a connection to the first and second reservoirs, and (iii) channels forming the connections, wherein the channels connecting the wells to reservoirs do not intersect to allow mixing of reagents or solvents provided from the reservoirs prior to such reagents or solvents being deposited in wells; b) providing a binding moiety bound with each said well which binding moieties bind a ligand that is present or prospectively present in a sample with which the well will be contacted, wherein at least two different binding moieties are provided in separate said wells; c) moving a sample from a reservoir into each of the wells to contact the bound binding ligand; d) parallelly pumping fluids from the connected first and second reservoirs through the wells to develop a detectable result that varies depending on the amount of the ligand present in the wells that binds to the binding moiety bound to the respective well; and e) measuring the detectable result. - View Dependent Claims (13, 14, 15, 16, 17, 18)
-
Specification