Appetite supression factor and related methods

• US 5,858,967 A
• Filed: 09/19/1997
• Issued: 01/12/1999
• Est. Priority Date: 01/20/1995
• Status: Expired due to Fees

• Current Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company), University of Washington
• Original Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company), University of Washington
• Inventors: Kuijper, Joseph L., Forstrom, John W., Weigle, David S.
• Primary Examiner(s): Carlson, Karen Cochrane
• Application Number:
  US08/938,001
• Time in Patent Office: 480 Days
• Field of Search: 424/78.1, 424/574, 435/69.1, 435/70.1, 435/71.1, 435/514, 2/536, 536/23.5
• US Class Current: 514/4.9
• CPC Class Codes: C07K 14/5759 Products of obesity genes, e.g. leptin, obese (OB), tub, fat

Abstract

Methods for identifying factors that regulate appetite are disclosed. Preferably, the methods feature determinination of decreased food consumption in selected recipient mammals following administration of a test sample. A product having appetite suppressing activity is also described. In addition, methods for reducing food consumption in a mammal, featuring administration of a pharmaceutical composition comprising a representative appetite suppression factor, are disclosed.

Claims

1. A method for inhibiting food consumption in a mammal, comprising administering an effective dose of a composition comprising an appetite suppression factor expressed by adipose tissue, wherein the factor is between about 14 kDa and 16 kDa in size as determined by SDS polyacrylamide gel electrophoresis under reducing conditions.

2. The method of claim 1, wherein the factor is essentially of a form obtainable by culturing a mammalian cell ex vivo under conditions wherein the factor is synthesized and secreted by the cell.

3. The method of claim 1, wherein the factor is obtained from adipose tissue.

4. The method of claim 1, wherein the factor is obtained from a cell containing an expression vector encoding the factor.

5. The method of claim 4, wherein the cell is a BHK cell or a CHO cell.

6. The method of claim 4, wherein the cell is a yeast cell.

7. The method of claim 1, wherein the factor is isolated.

8. A method for inhibiting food consumption in a mammal, comprising administering an effective dose of a composition comprising an appetite suppression factor expressed by adipose tissue, wherein the factor is ob protein.

9. The method of claim 8, wherein the factor is essentially of a form obtainable by culturing a mammalian cell ex vivo under conditions wherein the factor is synthesized and secreted by the cell.

10. The method of claim 8, wherein the factor is obtained from adipose tissue.

11. The method of claim 8, wherein the factor is obtained from a cell containing an expression vector encoding the factor.

12. The method of claim 11, wherein the cell is a BHK cell or a CHO cell.

13. The method of claim 11, wherein the cell is a yeast cell.

14. The method of claim 11, wherein the factor is isolated.