Hypersensitive response induced resistance in plants

• US 5,859,324 A
• Filed: 03/17/1997
• Issued: 01/12/1999
• Est. Priority Date: 06/07/1995
• Status: Expired due to Term

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Wei, Zhong-Min, Beer, Steven V.
• Primary Examiner(s): Tsang, Cecilia J.
• Assistant Examiner(s): HARLE, JENNIFER I
• Application Number:
  US08/819,539
• Time in Patent Office: 666 Days
• Field of Search: 514/2, 424/93, 435/800, 435/847, 800/200
• US Class Current: 800/298
• CPC Class Codes: A01N 61/00 Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/27 Pseudomonas
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: C07K 14/27 Erwinia (G)
• CPC Class Codes: C12N 15/8281 for bacterial resistance
• CPC Class Codes: C12N 15/8282 for fungal resistance
• CPC Class Codes: Y10S 435/80 Elimination or reduction of contamination by undersired ferments, e.g. aseptic cultivation
• CPC Class Codes: Y10S 435/847 Erwinia

Abstract

The present invention relates to a method of imparting pathogen resistance to plants. This involves applying a hypersensitive response elicitor polypeptide or protein in a non-infectious form to a plant under conditions where the polypeptide or protein contacts cells of the plant. The present invention is also directed to a pathogen resistant plant and a composition for imparting pathogen resistance to plants.

Claims

1. A pathogen-resistant plant to which a hypersensitive response eliciting bacterium, which does not cause disease in that plant, or a hypersensitive response eliciting polypeptide or protein has been externally applied, wherein the hypersensitive response eliciting polypetide or protein corresponds to that derived from a pathogen selected from the group consisting of Erwinia amylovora, Erwinia chrysanthemi, Pseudomonas syringae, Pseudomonas solancearum, Xanthamonas campestris, and mixtures thereof.

2. A pathogen-resistant plant according to claim 1, wherein the hypersensitive response eliciting polypeptide or protein is in isolated form.

3. A pathogen-resistant plant according to claim 1, wherein the plant cells are in contact with bacteria which do not cause disease and are transformed with a gene encoding the hypersensitive response eliciting polypeptide or protein.

4. A pathogen-resistant plant according to claim 1, wherein the plant cells are in contact with bacteria which do not cause disease in the plant, but do cause disease in other plant species, and contain a gene encoding the hypersensitive response eliciting polypeptide or protein.

5. A pathogen-resistant plant according to claim 2, wherein the hypersensitive response eliciting polypeptide or protein corresponds to that derived from Erwinia chrysanthemi.

6. A pathogen-resistant plant according to claim 5, wherein the hypersensitive response eliciting polypeptide or protein has an amino acid sequence corresponding to SEQ. ID. No. 1.

7. A pathogen-resistant plant according to claim 2, wherein the hypersensitive response eliciting polypeptide or protein corresponds to that derived from Erwinia amylovora.

8. A pathogen-resistant plant according to claim 7, wherein the hypersensitive response eliciting polypeptide or protein has an amino acid sequence corresponding to SEQ. ID. No. 3.

9. A pathogen-resistant plant according to claim 2, wherein the hypersensitive response eliciting polypeptide or protein corresponds to that derived from Pseudomonas syringae.

10. A pathogen-resistant plant according to claim 9, wherein the hypersensitive response eliciting polypeptide or protein has an amino acid sequence corresponding to SEQ. ID. No. 5.

11. A pathogen-resistant plant according to claim 2, wherein the hypersensitive response eliciting polypeptide or protein corresponds to that derived from Pseudomonas solanacearum.

12. A pathogen-resistant plant according to claim 11, wherein the hypersensitive response eliciting polypeptide or protein has an amino acid sequence corresponding to SEQ. ID. No. 7.

13. A pathogen-resistant plant according to claim 2, wherein the hypersensitive response eliciting polypeptide or protein corresponds to that derived from Xanthomonas campestris.

14. A pathogen-resistant plant according to claim 13, wherein the hypersensitive response eliciting polypeptide or protein has an amino acid sequence corresponding to SEQ. ID. No. 9.

15. A pathogen-resistant plant according to claim 2, wherein the plant is selected from the group consisting of dicots and monocots.

16. A pathogen-resistant plant according to claim 15, wherein the plant is selected from the group consisting of rice, wheat, barley, rye, cotton, sunflower, peanut, potato, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, strawberry, grape, raspberry, pineapple, soybean, tobacco, tomato, sorghum, and sugarcane.

17. A pathogen-resistant plant according to claim 15, wherein the plant is selected from the group consisting of Arabidopsis thaliana, Saintpaulia, petunia, pelangonium, poinsettia, chrysanthemum, carnation, and zinnia.

18. A pathogen-resistant plant according to claim 2, wherein the pathogen to which the plant is resistant is selected from the group consisting of a virus, bacterium, fungus, and combinations thereof.