Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same
First Claim
1. An array of oligonucleotide probes immobilized on a solid support, the array having at least four sets of oligonucleotide probes at least 9 nucleotides in length,(1) a first probe set having a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus, each probe being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide and each probe having an interrogation position occupied by a complementary nucleotide to the corresponding nucleotide,(2) three additional probe sets, each of which has a corresponding probe for each probe in the first probe set, the three corresponding probes in the three additional probe sets being identical to the corresponding probe from the first probe set or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes;
- provided the array lacks a complete set of oligonucleotide probes of a given length.
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Accused Products
Abstract
The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.
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Citations
26 Claims
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1. An array of oligonucleotide probes immobilized on a solid support, the array having at least four sets of oligonucleotide probes at least 9 nucleotides in length,
(1) a first probe set having a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus, each probe being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide and each probe having an interrogation position occupied by a complementary nucleotide to the corresponding nucleotide, (2) three additional probe sets, each of which has a corresponding probe for each probe in the first probe set, the three corresponding probes in the three additional probe sets being identical to the corresponding probe from the first probe set or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes; provided the array lacks a complete set of oligonucleotide probes of a given length. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 22, 23, 24, 25)
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15. A method of comparing a target nucleic acid from a human immunodeficiency virus with a reference sequence from a second human immunodeficiency virus having a predetermined sequence of nucleotides, the method comprising:
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(a) hybridizing the target nucleic acid to an array of oligonucleotide probes immobilized on a solid support, the array comprising oligonucleotide probes at least 9 nucleotides in length, the oligonucleotide probes comprising at least four sets of probes, (1) the first probe set having a probe corresponding to each nucleotide in a reference sequence, each probe being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide, and each probe having an interrogation position occupied by a complementary nucleotide to the corresponding nucleotide in the reference sequence, and (2) three additional probe sets, each of which has a corresponding probe for each probe in the first probe set, the three corresponding probes in the three additional probe sets being identical to the corresponding probe from the first probe set or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes (b) comparing the relative specific binding of four corresponding probes from the first, second, third and fourth probe sets to determine the identity of the corresponding nucleotide in the target sequence, (c) repeating (b) by comparing the relative specific binding of a further four corresponding probes until the identity of each nucleotide of interest in the target sequence has been determined thereby indicating whether the target sequence is the same or different from the reference sequence. - View Dependent Claims (16, 17, 18, 19, 20, 21, 26)
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Specification