Use of stem cell factor and soluble interleukin-6 receptor for the ex vivo expansion of hematopoietic multipotential cells
First Claim
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1. A method for the ex vivo expansion of multipotential hematopoietic cells, comprising culturing the cells in a medium comprising soluble interleukin-6 receptor, interleukin-6, and stem cell factor, said cells, interleukin-6 receptor, interleukin-6, and stem cell factor derived from a human species, said interleukin-r receptor, interleukin-6, and stem cell factor being present in amounts effective to produce a mature erythroid cell population in the absence of erythropoietin.
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Abstract
Stem cell factor in combination with soluble interleukin-6 receptor, interleukin-6, for gp130 signaling, supports the proliferation, differentiation and terminal maturation of blood cells from normal human hematopoietic multipotential cells.
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8 Claims
- 1. A method for the ex vivo expansion of multipotential hematopoietic cells, comprising culturing the cells in a medium comprising soluble interleukin-6 receptor, interleukin-6, and stem cell factor, said cells, interleukin-6 receptor, interleukin-6, and stem cell factor derived from a human species, said interleukin-r receptor, interleukin-6, and stem cell factor being present in amounts effective to produce a mature erythroid cell population in the absence of erythropoietin.
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8. A method generating differentiated blood cell colonies by ex vivo expansion of multipotential hematopoietic cells, comprising culturing the hematopoietic cells in a medium comprising soluble interleukin-6 receptor, interleukin-6, and stem cell factor, said hematopoietic cells, interleukin-6 receptor, interleukin-6, and stem cell factor derived from a human species, said interleukin-6 recaptor, interleukin-6, and stem cell factor being present in amounts effective to produce a mature erythroid cell population in the absence of erythropoietin.
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