Solutions containing mismatch repair system proteins mutL and mutS

• US 5,861,482 A
• Filed: 06/02/1995
• Issued: 01/19/1999
• Est. Priority Date: 05/12/1989
• Status: Expired due to Term

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Modrich, Paul L., Su, Shin-San, Lahue, Robert S., Cooper, Deani Lee, Au, Karin G., Worth, Leroy Jr.
• Primary Examiner(s): KEMMERER, ELIZABETH
• Application Number:
  US08/458,733
• Time in Patent Office: 1,327 Days
• Field of Search: 530/380, 435/193, 435/183
• US Class Current: 530/350
• CPC Class Codes: C07K 14/245 Escherichia (G)
• CPC Class Codes: C12N 15/00 Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00
• CPC Class Codes: C12N 15/102 Mutagenizing nucleic acids
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 2521/514 Mismatch repair protein
• CPC Class Codes: C12Q 2535/131 Allele specific probes
• CPC Class Codes: C12Q 2537/113 Heteroduplex formation
• CPC Class Codes: C12Y 302/0202 DNA-3-methyladenine glycosylase I (3.2.2.20), i.e. adenine DNA glycosylase
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A diagnostic method for detecting a base pair mismatch in a DNA duplex, comprising the steps of contacting at least one strand of a first DNA molecule with the complementary strand of a second DNA molecule under conditions such that base pairing occurs contacting a DNA duplex potentially containing a base pair mismatch with a mispair recognition protein under conditions suitable for the protein to form a specific complex only with the DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and detecting any complex as a measure of the presence of a base pair mismatch in the DNA duplex.

Claims

1. A solution comprising the MutL and MutS proteins of Escherichia coli and no other protein of a mismatch repair system.

2. A solution comprising homologs of the MutL and MutS proteins of Escherichia coli and no other protein of a mismatch repair system.