Parallel reaction cassette and associated devices
First Claim
Patent Images
1. A device for conducting parallel reactions, comprising:
- (a) a cassette formed of a body having an upper surface and a lower surface and including an upper film or a lower film attached to the upper or lower surface, respectively, wherein the upper or lower film is formed of a flexible material;
(b) two or more reaction flow-ways in the cassette, wherein each reaction flow-way comprises two or more fluid chambers which comprise a first supply chamber and a first reaction chamber having an upper wall and a lower wall, and wherein the fluid chambers are serially connected by first fluid exchange channels;
(c) a valve for controlling the flow of fluid through a first fluid exchange channel;
(d) a pump for moving fluids into or out of the fluid chambers; and
(e) a first inlet port on the cassette connected to a first supply chamber in each reaction flow-way by a second fluid exchange channel.
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Abstract
The invention provides a parallel reaction device for conducting reactions therein comprising one or more reaction flow-ways, each such reaction flow-way comprising one or more chambers connected serially by fluid exchange channels, additional fluid exchange channels connecting such reaction channels in parallel, valve means for initiating and impeding the flow of fluids through such fluid exchange channels, and means for moving the flow of fluids into and out of such chambers.
897 Citations
21 Claims
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1. A device for conducting parallel reactions, comprising:
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(a) a cassette formed of a body having an upper surface and a lower surface and including an upper film or a lower film attached to the upper or lower surface, respectively, wherein the upper or lower film is formed of a flexible material; (b) two or more reaction flow-ways in the cassette, wherein each reaction flow-way comprises two or more fluid chambers which comprise a first supply chamber and a first reaction chamber having an upper wall and a lower wall, and wherein the fluid chambers are serially connected by first fluid exchange channels; (c) a valve for controlling the flow of fluid through a first fluid exchange channel; (d) a pump for moving fluids into or out of the fluid chambers; and (e) a first inlet port on the cassette connected to a first supply chamber in each reaction flow-way by a second fluid exchange channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A device for conducting assays in parallel using fluids that are confined to a disposable cassette comprising:
the disposable assay cassette, which comprises; (i) at least two reaction flow-ways, including a first reaction flow-way designed to receive and assay an experimental sample and a second reaction flow-way designed to receive and assay a negative control, (ii) for each reaction flow-way, at least one supply chamber connected thereto and containing fluids needed in the assay and at least one reaction chamber, (iii) a negative control supply chamber connected with the second reaction flow-way and containing the negative control, and (iv) a test sample supply chamber connected with the first reaction flow-way designed to receive a test sample through an inlet connected with the test sample supply chamber, valves for controlling the flow of fluids in the cassette, and an instrument comprising a temperature control unit for controlling in parallel the temperature in a reaction chamber in each reaction flow-way, valve actuators for opening and closing the valves in the cassette, and one or more pumps for pushing fluid out of the various supply chambers and reaction chambers of the cassette. - View Dependent Claims (18, 19, 20, 21)
Specification