Culture vessel assembly
First Claim
1. An assembly comprising:
- a test plate comprising a plurality of wells having openings therein for receiving tissue culture media or cell culture inserts and upstanding sidewall forming an outside border of said plate;
a lid removably positioned on said plate over said wells, comprising a substantially planar cover extending over said wells comprising an upper surface and a lower surface, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly having an inner and outer surface;
an inner perimeter area on said lid between said inner surface of said cover and said inner surface of said skirt;
a sealing material in said perimeter area of said lid;
a plurality of orifices in said planar cover each comprising a gas permeable membrane and positioned whereby said orifices are in direct alignment with said wells of said test plate; and
means for removably securing said lid with said plate.
2 Assignments
0 Petitions
Accused Products
Abstract
A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for accommodating a cell culture insert and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured in the cell culture insert. The means for varying the gas diffusion is a gas permeable membrane provided in a plurality of openings in the lid and a plurality of removable thin impermeable film labels are provided for selectively occluding passage of gases through the gas permeable membranes. The means for minimizing biological contamination of the cells being cultured is a sealing material located in the lid and means for removably securing the lid and the plate together whereby the sealing material is compressed between the lid and the plate.
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Citations
26 Claims
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1. An assembly comprising:
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a test plate comprising a plurality of wells having openings therein for receiving tissue culture media or cell culture inserts and upstanding sidewall forming an outside border of said plate; a lid removably positioned on said plate over said wells, comprising a substantially planar cover extending over said wells comprising an upper surface and a lower surface, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly having an inner and outer surface; an inner perimeter area on said lid between said inner surface of said cover and said inner surface of said skirt; a sealing material in said perimeter area of said lid; a plurality of orifices in said planar cover each comprising a gas permeable membrane and positioned whereby said orifices are in direct alignment with said wells of said test plate; and means for removably securing said lid with said plate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A lid for use in conjunction with a tissue culture plate comprising:
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a substantially planar cover comprising an upper surface and a lower surface; a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly having an inner and outer surface; an inner perimeter area in said lid between said upper surface of said cover and said inner surface of said skirt; a sealing material in said perimeter area; a plurality of orifices in said cover each comprising a gas permeable membrane; a gas impermeable film removably attached to said cover over said orifice and said gas permeable membrane whereby said film may be fully or partially removed; and a lever on said outer surface of said skirt. - View Dependent Claims (14, 15)
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16. A device for growing cells or tissue culture in vitro comprising:
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a body comprising an upper surface, a lower surface, a plurality of wells substantially disposed between said upper and lower surface and upstanding sidewall forming an outside border of said body; a removable lid, comprising a top wall and a peripheral skirt that extends from said top wall and means for allowing gas diffusion into and out of said device through said top wall, which is in alignment with said wells of said body wherein said means for allowing gas diffusion into and out of the device is a plurality of orifices and a gas permeable membrane associated with said orifices and in direct alignment with said wells of said body; and a plurality of gas impermeable films removably attached to said cover over said orifices, whereby said films may be partially or fully removed to assist with gas diffusion in said device. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of culturing cells comprising:
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(a) providing a culture vessel comprising a test plate comprising a plurality of wells having openings therein for receiving tissue culture media or a cell culture insert and upstanding sidewall forming an outside border of said plate;
at least one cell culture insert positioned in said well of said test plate, a lid removably positioned over said plate comprising a substantially planar cover extending over said wells, a skirt surrounding said cover defining a plurality of corners on said cover and extending downwardly in spaced relation with respect to said sidewall of said plate, an inner perimeter area on said lid between said cover and said skirt;
a sealing material in said perimeter area of said lid;
a plurality of orifices in said planar cover each comprising a gas permeable membrane wherein said orifices are in direct alignment with said wells of said plate;
gas impermeable films removably attached to said cover over each of said orifices; and
means for removably securing said lid with said plate comprising a lever on said skirt of said lid and a receiving handle for said lever on said sidewall of said plate;(b) removing said lid from said plate by applying a horizontal force to said lever towards said skirt of said lid and sliding said lever from said receiving handle and lifting said lid from said plate; (c) placing cell inserts comprising a membrane within said wells of said culture vessel; (d) depositing a layer of cells on each membrane of each of said cell inserts within said culture vessel; (e) overlaying the layer of cells with a growth medium; (f) securing said lid over said plate by applying a horizontal force to said lever towards said skirt of said lid and sliding said lever into said receiving handle on said plate; and (g) directly ventilating said membrane of each said cell insert by permitting air access thrusts through said orifices in said lid by partially or fully removing and re-attaching said labels over said orifices.
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Specification