Automated nucleic acid isolation
First Claim
1. A device for nucleic acid extraction from one or more biological samples, comprising a housing having a slot and a removable chemistry cassette, wherein the cassette fits into the slot, wherein the cassette has attached thereto a separable sample transfer/storage strip for holding and storing nucleic acids extracted from a biological sample, and wherein the cassette includes reagents suitable for nucleic acid extraction.
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Abstract
The present invention relates to a device and a method of use for that device for the automated preparation of nucleic acid from a biological sample. The device relates to a cassette in which all chemical manipulations occur, which includes a storage/transfer strip where the prepared nucleic acid is deposited and stored without contamination. Moreover, the waste of the preparation protocol is contained in the cassette, which, upon the separation of the filled storage/transfer strip is completely sealed off from the environment. In a preferred embodiment, the inventive device can prepare nucleic acid preparations from up to 24 different biological samples at a time.
558 Citations
43 Claims
- 1. A device for nucleic acid extraction from one or more biological samples, comprising a housing having a slot and a removable chemistry cassette, wherein the cassette fits into the slot, wherein the cassette has attached thereto a separable sample transfer/storage strip for holding and storing nucleic acids extracted from a biological sample, and wherein the cassette includes reagents suitable for nucleic acid extraction.
Specification