Multichannel, multipurpose sample collection and drug delivery system for laboratory animals
First Claim
1. A sample collection device comprising:
- a) a coil of flexible tubing, one end for coupling to a sample site;
b) a timer; and
c) a pump coupled to the other one end of the coil, wherein at preset intervals controlled by said timer said pump draws a sample from the sample site into the coil followed by a bubble of gas to keep successive samples separate in the coil until the samples are removed for analysis.
2 Assignments
0 Petitions
Accused Products
Abstract
A multichannel, multipurpose sample collection and drug delivery system for laboratory animals includes several sampling and/or infusion devices mounted on a powered turntable above the cage but out of the animal'"'"'s sight. Probes are constructed using a unique four-channel design: this permits (a) the periodic infusion of drugs without the possibility of artifacts due to pressure shocks and (b) the addition of preservatives to the sample at the site of its collection. A simplified sample collection device stores samples in a coil of PTFE tubing, and a constant-pressure perfusion system serves microdialysis probes. Further advantageous embodiments of the present invention include (a) systems to measure feeding, drinking and motor activity; (b) automated and unattended systems for blood withdrawal and drug delivery; (c) a data link with a personal computer to provide for data acquisition and more sophisticated control; (d) an improved method for sampling extracellular concentrations of neuropeptides, which are difficult to measure with conventional microdialysis; (e) the capability to perform electrophysiology simultaneously with microdialysis (or other sampling methods); and (f) the adaptation of the system for use with animals larger or smaller than the rat.
31 Citations
18 Claims
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1. A sample collection device comprising:
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a) a coil of flexible tubing, one end for coupling to a sample site; b) a timer; and c) a pump coupled to the other one end of the coil, wherein at preset intervals controlled by said timer said pump draws a sample from the sample site into the coil followed by a bubble of gas to keep successive samples separate in the coil until the samples are removed for analysis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An automated fluid sampling system for obtaining samples from a living organism comprising:
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a) a coil of tubing; b) an indwelling cannula attached to the living organism, and also attached to one end of the coil, said indwelling cannula having a main cannula with a tip, a body and an concentric inner cannula; c) a first syringe attached to the other end of the coil; d) a second syringe filled with an anticoagulant and being coupled to the indwelling cannula via the concentric inner cannula, wherein said second syringe delivers said anticoagulant via the concentric inner cannula to a point about 1 mm from the tip of the main cannula; e) a third syringe being coupled to the indwelling cannula via the body of the indwelling cannula, and delivering a preservative solution into the body of the cannula; f) an external computer transmitting an activation signal; g) three stepper motors, one coupled to each of the syringes; and h) a microcomputer receiving the activation signal from the external computer and controlling the three stepper motors according to the activation signal. - View Dependent Claims (17, 18)
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Specification