Gas driven gene delivery instrument

• US 5,865,796 A
• Filed: 12/16/1996
• Issued: 02/02/1999
• Est. Priority Date: 01/21/1994
• Status: Expired due to Term

• Current Assignee: PowderJect Vaccines, Inc. (Bayer AG)
• Original Assignee: PowderJect Vaccines, Inc. (Bayer AG)
• Inventors: McCabe, Dennis E.
• Primary Examiner(s): MCDERMOTT, CORRINE MARIE
• Application Number:
  US08/767,374
• Time in Patent Office: 778 Days
• Field of Search: 604/23, 604/24, 604/62, 604/68-72, 604/57-59, 604/131, 604/140, 604/141, 604/143, 604/146, 604/147, 128/200.14, 128/200.21, 128/200.22, 128/200.23, 239/311, 239/338, 239/346
• US Class Current: 604/71
• CPC Class Codes: A61M 2005/005 Magazines with multiple ampoules directly inserted into an injection or infusion device, e.g. revolver-like magazines containing ampoules with or without needles
• CPC Class Codes: A61M 5/2053 Media being expelled from injector by pressurised fluid or vacuum for infusion A61M5/145, A61M5/155
• CPC Class Codes: A61M 5/3015 for injecting a dose of particles in form of powdered drug, e.g. mounted on a rupturable membrane and accelerated by a gaseous shock wave or supersonic gas flow cell injection devices C12M3/006
• CPC Class Codes: C12M 35/00 Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87

Abstract

A gas driven apparatus for accelerating particles coated with a genetic material into a target comprises a reservoir for releasably retaining a gas at a sufficiently high pressure to detach the particles from the surface of a sample cartridge and to carry the particles through the apparatus toward the target. When leaving the apparatus, the particles entrained in the gas stream pass through a substantially conical exit nozzle which causes the pattern of distribution of the particles to greatly expand. Methods for using the apparatus and for preparing the sample cartridges are also described.

Claims

1. A gene delivery instrument comprising:

a body having a particle acceleration passage formed therein, said acceleration passage having an opening at one end thereof,

means for connecting the acceleration passage with a source of compressed gas to selectively admit gas into said passage, thereby forming an accelerating gas stream;

a cartridge chamber adapted to accept and retain a particle cartridge containing carrier particles coated with a genetic material, said cartridge chamber arranged within the acceleration passage such that the accelerating gas stream passing through the acceleration passage picks up and accelerates carrier particles from the cartridge; and

an exit nozzle at the opening of the acceleration passage, said exit nozzle widening substantially away from the body wherein the widening portion of the nozzle is longer than it is wide.

2. The instrument of claim 1, wherein the means for connecting the acceleration passage with a source of compressed gas comprises a valve which controls the flow of gas from the source to the acceleration passage.

3. A gene delivery instrument comprising:

a body having a particle acceleration passage formed therein, said acceleration passage having an opening at one end thereof;

means for connecting the acceleration passage with a source of compressed gas to selectively admit gas into said passage, thereby forming an accelerating gas stream;

a cylindrical cartridge chamber adapted to accept and retain a tubular particle cartridge which contains on the interior surface thereof carrier particles coated with a genetic material, said cartridge chamber arranged within the body and communicating with the acceleration passage such that the accelerating gas stream passes through the particle cartridge to pick up and accelerate carrier particles from the cartridge; and

an exit nozzle at the opening of the acceleration passage, said exit nozzle widening substantially away from the body wherein the widening portion of the nozzle is longer than it is wide.

4. The instrument of claim 3, wherein the means for connecting the acceleration passage with a source of compressed gas comprises a valve which controls the flow of gas from the source to the acceleration passage.

5. A particle cartridge for use in a compressed gas-driven gene delivery instrument, said cartridge comprising a concave body having an arcuate passage formed on a surface thereof wherein said arcuate passage contains a deposit of carrier particles coated with genetic material.