Bifunctional crown ether-based cation-exchange stationary phase for liquid chromatography

• US 5,865,994 A
• Filed: 03/13/1997
• Issued: 02/02/1999
• Est. Priority Date: 03/13/1997
• Status: Expired due to Term

• Current Assignee: Dionex Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Dionex Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Jagodzinski, Jacek, Riviello, John, Pohl, Christopher, Rey, Maria
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US08/816,878
• Time in Patent Office: 691 Days
• Field of Search: 210/635, 210/656, 210/198.2, 210/502.1, 428/403, 428/407, 428/401, 502/402
• US Class Current: 210/198.2
• CPC Class Codes: B01D 15/362 Cation-exchange
• CPC Class Codes: B01J 39/26 Cation exchangers for chromatographic processes
• CPC Class Codes: B01J 45/00 Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties ion-exchange chromatography processes B01D15/36

Abstract

In accordance with the present invention, an improved bifunctional cation-exchange composition has been formed which comprises synthetic resin support particles and both crown ether and non-crown ether functional groups attached thereto. The compositions of the present invention provide for novel, enhanced cation separation capabilities and, depending upon the type of functional groups employed, permit effective separation of cations which elute at approximately the same time off standard and commonly used cation-exchange columns. The presently described bifunctional cation-exchange compositions also permit the effective separation of one cation from a mixture comprising another cation that is present at a significantly greater concentration. Formed articles and methods employing the bifunctional cation-exchange composition are also provided.

Claims

1. A bifunctional cation-exchange composition for use in cation-exchange chromatography comprising:

(a) synthetic resin support particles;

(b) crown ether functional groups attached to said synthetic resin support particles, wherein said crown ether functional groups are capable of forming complexes with at least one cation present in an eluent in contact therewith, thereby delaying the elution of said at least one cation from said bifunctional cation-exchange composition, and

(c) non-crown ether cation-exchange functional groups attached to said synthetic resin support particles, wherein said non-crown ether cation-exchange functional groups are capable of interacting with cations present in said eluent.

2. The bifunctional cation-exchange composition according to claim 1, wherein said non-crown ether cation-exchange functional groups are selected from the group consisting of sulfonate groups, carboxylate groups, phosphonate groups or a mixture thereof.

3. The bifunctional cation-exchange composition according to claim 2, wherein said mixture is a mixture of carboxylate and phosphonate groups.

4. The bifunctional cation-exchange composition according to claim 2, wherein said non-crown ether cation-exchange functional groups are carboxylate groups.

5. The bifunctional cation-exchange composition according to claim 1 which comprises at least two different non-crown ether cation-exchange functional groups attached to said synthetic resin support particles.

6. The bifunctional cation-exchange composition according to claim 1, wherein said crown ether functional groups have a higher affinity for complexing with a single specific cation present in said eluent as compared to any other cation present in said eluent, said single specific cation being selected from the group consisting of K⁺, Na⁺, Li⁺, NH₄⁺, Ca⁺⁺, Mg⁺⁺, Sr⁺⁺ and Ba⁺⁺.

7. The bifunctional cation-exchange composition according to claim 6, wherein said single specific cation is K⁺ cation.

8. The bifunctional cation-exchange composition according to claim 6, wherein said single specific cation is Na⁺ cation.

9. The bifunctional cation-exchange composition according to claim 6, wherein said single specific cation is Li⁺ cation.

10. The bifunctional cation-exchange composition according to claim 6, wherein said single specific cation is NH₄⁺ cation.

11. The bifunctional cation-exchange composition according to claim 1 which comprises at least two different crown ether functional groups attached to said synthetic resin support particles.

12. The bifunctional cation-exchange composition according to claim 11, wherein said at least two different crown ether functional groups each exhibit a different affinity for complexation with at least one cation.

13. The bifunctional cation-exchange composition according to claim 1, wherein said crown ether functional groups are attached to said synthetic resin support particles through a linker chain of from about 1 to about 20 atoms in length.

14. The bifunctional cation-exchange composition according to claim 13, wherein each of said about 1 to about 20 atoms in said linker chain is selected from the group consisting of substituted or unsubstituted carbon, oxygen, nitrogen or sulfur atoms.

15. The bifunctional cation-exchange composition according to claim 14, wherein each of said about 1 to about 20 atoms in said linker chain are substituted or unsubstituted carbon atoms.

16. The bifunctional cation-exchange composition according to claim 15, wherein said carbon atoms are hydrogen saturated.

17. The bifunctional cation-exchange composition according to claim 13, wherein at least one of said about 1 to about 20 atoms in said linker chain is oxygen, nitrogen or sulfur.

18. The bifunctional cation-exchange composition according to claim 13, wherein at least one atom in said linker chain is substituted with a group selected from the group consisting of alkyl, alkoxyl, carboxy, carboxyalkyl, hydroxyalkyl or hydroxyl.

19. The bifunctional cation-exchange composition according to claim 1, wherein said crown ether functional groups are selected from the group consisting of 9-crown-3, 12-crown-4, 15-crown-5, 18-crown-6, 21-crown-7, 24-crown-8, 27-crown-9 and 30-crown-10.

20. The bifunctional cation-exchange composition according to claim 19, wherein said crown ether functional group is 18-crown-6.

21. A chromatography column for use in cation-exchange chromatography comprising the bifunctional cation-exchange composition according to claim 1.