Method of producing an antibody with a peptide corresponding to membrane-bound IgA

• US 5,866,129 A
• Filed: 11/04/1991
• Issued: 02/02/1999
• Est. Priority Date: 06/21/1989
• Status: Expired due to Fees

• Current Assignee: Genentech, Inc. (Roche Holding AG)
• Original Assignee: Tanox Biosystems Inc. (Roche Holding AG)
• Inventors: Chang, Nancy T., Chang, Tse Wen
• Primary Examiner(s): Fitzgerald, David L.
• Assistant Examiner(s): Kaufman, Claire M.
• Application Number:
  US07/785,565
• Time in Patent Office: 2,647 Days
• Field of Search: 530/387, 530/300, 530/350, 530/387.3, 530/388.73, 530/412, 514/12, 514/2, 424/185.1
• US Class Current: 424/185.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 47/643 Albumins, e.g. HSA, BSA, ovalbumin or a Keyhole Limpet Hemocyanin [KHL]
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/4241 against anti-human or anti-animal Ig
• CPC Class Codes: C07K 16/4283 against an allotypic or isotypic determinant on Ig
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: Y10S 530/861 involving IgG3, IgG4, IgA, or IgY
• CPC Class Codes: Y10S 530/866 involving immunoglobulin or antibody fragment, e.g. fab', fab, fv, fc, heavy chain or light chain
• CPC Class Codes: Y10S 530/867 involving immunoglobulin or antibody produced via recombinant dna technology
• CPC Class Codes: Y10S 530/868 involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance

Abstract

The invention relates to a method of using a peptide which has the sequence of epitopes which are present on B cell-bound but not secreted IgA. This induces production of the antibody itself. These extracellular peptide segments form, entirely or in part, antigenic epitopes unique to membrane-bound but not secreted IgA.

Claims

1. A method of using a peptide which includes an amino acid sequence which is the same as the extracellular segment of the membrane-bound domain of IgA or an epitope thereof, said method comprising immunizing an animal with said peptide to produce an antibody which binds specifically to the extracellular segment of the membrane-bound domain of human IgA on the surface of B-cells and which antibody does not bind to secreted, soluble IgA, and isolating said antibody.

2. A method of using a peptide which includes the amino acid sequence of SEQ ID NO:

1 or an epitope thereof, comprising immunizing an animal to produce antibodies which binds specifically to the extracellular segment of the membrane-bound domain of human IgA on the surface of B-cells and which antibody does not bind to secreted, soluble IgA, and isolating said antibody.