Apparatus for the detection of an analyte utilizing mesoscale flow systems
DCFirst Claim
1. A device for detecting the presence of an analyte in a fluid sample, the device comprising:
- a solid substrate microfabricated to define;
a sample inlet port;
a mesoscale flow system comprising;
a sample flow channel in fluid communication with said inlet port; and
an analyte detection region in fluid communication with said flow channel comprising a binding moiety immobilized therein for specifically binding said analyte, said detection region having a mesoscale dimension; and
a detection window disposed in said detection region for transmitting a signal indicating binding of said analyte to said binding moiety to a detection means disposed adjacent to said window.
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Abstract
Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.
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Citations
18 Claims
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1. A device for detecting the presence of an analyte in a fluid sample, the device comprising:
a solid substrate microfabricated to define; a sample inlet port; a mesoscale flow system comprising; a sample flow channel in fluid communication with said inlet port; and an analyte detection region in fluid communication with said flow channel comprising a binding moiety immobilized therein for specifically binding said analyte, said detection region having a mesoscale dimension; and a detection window disposed in said detection region for transmitting a signal indicating binding of said analyte to said binding moiety to a detection means disposed adjacent to said window. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A device for detecting the presence of an analyte in a fluid sample, the device comprising:
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a solid substrate; a sample flow channel fabricated into said substrate, said sample flow channel having at least one cross sectional dimension in a range of from 0.1 μ
m to 500 μ
m;an analyte binding region fabricated into said substrate and in fluid communication with said flow channel, said analyte binding region comprising a binding moiety immobilized on a surface of said substrate within said binding region, wherein said binding moiety specifically binds said analyte, at least one of said sample flow channel and said analyte binding region having at least one cross sectional dimension of from about 0.1 μ
m to about 500 μ
m;a cover disposed over said sample flow channel and said analyte binding region; a sample inlet port disposed through one of said substrate or said cover, said sample inlet being in fluid communication with said sample flow channel, and a detection window disposed in one of said substrate or said cover for transmitting a signal indicating binding of said analyte to said binding moiety to a detector disposed adjacent said detection window.
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Specification