Raman optrode processes and devices for detection of chemicals and microorganisms
First Claim
1. The process for any one of at least the detection, identification and quantification of one or more analytes by Raman spectroscopy comprising the steps of:
- providing a bioconcentrator which binds with at least one analyte,bringing into contact with said bioconcentrator at least one analyte to effect binding of the at least one analyte on to the bioconcentrator to form a medium to be analyzed consisting essentially of said bioconcentrator and said at least one analyte and wherein at least some of said at least one analyte is bound to said bioconcentrator,irradiating the said medium containing said bioconcentrator and the bound at least one analyte thereon with light from a source wherein said light produces Raman scattering from the irradiated bioconcentrator and the at least one analyte bound thereto, wherein the Raman scattering is not produced by a reporter attached to either the bioconcentrator or the analyte,collecting and processing the Raman scattering emanating from said bioconcentrator and the at least one analyte on said bioconcentrator, andanalyzing the processed Raman scattering to determine the presence and/or identity of the at least one analyte.
0 Assignments
0 Petitions
Accused Products
Abstract
A methodology and devices for detecting or monitoring or identifying chemical or microbial analytes are described. The methodology comprises four basic steps: (1) The gas or liquid medium to be monitored or analyzed is brought into contact with a bioconcentrator which is used to bind with or collect and concentrate one or more analytes. (2) The bioconcentrator-analyte complex is then exposed to radiation of one or more predetermined wavelengths to produce Raman scattering spectral bands. (3) At least a portion of the Raman spectral bands are collected and processed by a Raman spectrometer to convert the same into an electrical signal. And (4) the electrical signal is processed to detect and identify, qualitatively and/or quantitatively, the analyte(s). The methodology of this invention may also comprise Raman reactive capacity analysis of the bioconcentrator itself, simultaneously with or independently from the detection of the analyte, to determine the potential ability of the bioconcentrator to complex with analytes; the results of this latter analysis may be used to affect or alter or modify the methodology involved in detection and analysis of the analytes. Also the invention is accomplished by a Raman Optrode comprising: a bioconcentrator capable of binding with the analyte(s); a mechanism or procedure or device for bringing the gas or liquid sample into contact with the bioconcentrator; a light source suitable for generating Raman scattering; a Raman spectrometer capable of collecting and processing the Raman scattering spectral information and translating it into an electrical signal; and electronic hardware and software for analyzing the electrical signal and translating the signal into information on the presence, identity and/or quantity of the bound analytes. Various forms of bioconcentrators are described, as well as a variety of analytes which may be detected, monitored, or identified by this invention, and a variety of devices which can be fabricated based on this invention.
334 Citations
24 Claims
-
1. The process for any one of at least the detection, identification and quantification of one or more analytes by Raman spectroscopy comprising the steps of:
-
providing a bioconcentrator which binds with at least one analyte, bringing into contact with said bioconcentrator at least one analyte to effect binding of the at least one analyte on to the bioconcentrator to form a medium to be analyzed consisting essentially of said bioconcentrator and said at least one analyte and wherein at least some of said at least one analyte is bound to said bioconcentrator, irradiating the said medium containing said bioconcentrator and the bound at least one analyte thereon with light from a source wherein said light produces Raman scattering from the irradiated bioconcentrator and the at least one analyte bound thereto, wherein the Raman scattering is not produced by a reporter attached to either the bioconcentrator or the analyte, collecting and processing the Raman scattering emanating from said bioconcentrator and the at least one analyte on said bioconcentrator, and analyzing the processed Raman scattering to determine the presence and/or identity of the at least one analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
-
Specification