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Delivery of bioactive compounds to an organism

  • US 5,869,041 A
  • Filed: 09/13/1996
  • Issued: 02/09/1999
  • Est. Priority Date: 01/12/1996
  • Status: Expired due to Fees
First Claim
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1. A method of delivering a protein to a mammal comprising the steps of:

  • growing in vitro a plurality of cells from the same species as said mammal, wherein at least a subset of said cells comprise a foreign DNA sequence operably linked to a promoter and encoding a protein, and wherein said cells are mixed with an extracellular matrix to create a suspension;

    placing said suspension in a vessel having a three-dimensional geometry approximating the in vivo gross morphology of a tissue of interest, said vessel having attachment surfaces thereon; and

    allowing the suspension to coalesce;

    culturing said coalesced suspension under conditions in which said cells connect to said attachment surfaces and form an organized tissue having an in vivo-like gross and cellular morphology of said tissue of interest, and wherein the organized tissue further is comprised of post-mitotic cells; and

    implanting said tissue into said mammal, whereby said protein is produced and delivered to said mammal, whereby said protein is of a type or produced in an amount not produced by said tissue of interest lacking said foreign DNA sequence, wherein said protein is produced sufficiently to provide a therapeutic effect to the mammal upon implantation of said organized tissue into said mammal.

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