Procedure for the analysis of biological substances in a conductive liquid medium

• US 5,869,244 A
• Filed: 05/17/1996
• Issued: 02/09/1999
• Est. Priority Date: 07/07/1994
• Status: Expired due to Term

• Current Assignee: L'Ecole Centtrale De Lyon
• Original Assignee: L'Ecole Centtrale De Lyon
• Inventors: Souteyrand, Eliane, Martin, Jean-Rene, Mikkelsen, Susan Rosalie, Lawrence, Marcus Francois
• Primary Examiner(s): Campbell, Eggerton A.
• Application Number:
  US08/649,985
• Time in Patent Office: 998 Days
• Field of Search: 435/4, 435/6, 435/7.1, 205/157
• US Class Current: 435/6.11
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The present invention relates to a procedure for the qualitative and/or quantitative analysis of biological substances, which are preferably biological substances, that are present in a conductive liquid medium, with the aid of at least one affinity sensor that includes at least one structure that includes at least one semiconductor material, which is coated on one of its surface with at least one layer of an isolating material, which in turn is affixed adhesively to at least one sensitive membrane, which is in contact with the conductive medium and which includes ligands that are complementary to the biological substances in question and which are capable of, and suitable for, forming pairs specifically with the latter biological substances, with the said procedure being characterized by the fact that it consists essentially of applying a voltage between the semiconductor and the conductive medium; of gathering the variations in the electrical signals induced by a charge-effect phenomenon directly and essentially linked to the specific pairings of the biological substances with their complementary ligands included in the sensitive membrane; and interpreting the collected signals in order to identify and/or quantify the substances in question.

Claims

1. An affinity sensor for detecting a biological substance present in a conductive liquid medium, comprising:

at least one semiconductor/isolator/sensitive membrane, wherein said sensitive membrane comprises at least one nucleic acid capable of forming a specific pair with said biological substance, thereby causing a charge-effect which produces measurable variations in an electrical signal.

2. The affinity sensor of claim 1, wherein said semiconductor comprises silicon, said isolator comprises silicon dioxide, and said nucleic acid is affixed to said silicon dioxide.

3. The affinity sensor of claim 2, wherein said nucleic acid is affixed to said silicon dioxide by means of spacing compounds.