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Methods for detection of gram negative bacteria

  • US 5,869,272 A
  • Filed: 05/31/1995
  • Issued: 02/09/1999
  • Est. Priority Date: 09/18/1989
  • Status: Expired due to Term
First Claim
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1. Method for the determination of chlamydial or gram negative bacterial antigen comprising:

  • providing an optically active surface comprising an attachment layer and a layer of non-specific protein, said optically active surface exhibiting a first color in response to light impinging thereon, and exhibiting a second color comprising an intensity of at least one wavelength of light different from said first color, in response to said light when said antigen is present on said attachment layer,said attachment layer selected from the group of chemicals consisting of dendrimers, star polymers, molecular self-assembling polymers, polymeric siloxanes, and film forming latexes, provided on said optically active surface, which promote adhesion of the antigen to the optically active surface by hydrophobic interactions, andsaid layer of non-specific protein provided on said attachment layer,contacting said attachment layer with a sample potentially containing extracted antigen under conditions in which said antigen can bind to said attachment layer and can interact with said optically active surface to cause said optically active surface to exhibit said second color when said antigen is present, anddetecting said second color by use of an ellipsometer, a reflectometer, a comparison ellipsometer, a profilometer or a thin film analyzer as an indication of the presence of said antigen.

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