DNA encoding retinotic acid receptor epsilon

• US 5,869,284 A
• Filed: 06/06/1995
• Issued: 02/09/1999
• Est. Priority Date: 06/24/1994
• Status: Expired due to Term

• Current Assignee: Human Genome Sciences Incorporated (GSK plc)
• Original Assignee: Human Genome Sciences Incorporated (GSK plc)
• Inventors: Cao, Liang, Ni, Jian, Fleischmann, Robert D.
• Primary Examiner(s): Fitzgerald, David L.
• Assistant Examiner(s): Basham, Daryl A.
• Application Number:
  US08/466,120
• Time in Patent Office: 1,344 Days
• Field of Search: 536/23.5, 435/69.1, 435/172.3, 435/252.3, 530/350, 530/180, 535/1, 535/3, 535/10, 535/23, 535/55, 535/66, 930/180
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/70567 Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
• CPC Class Codes: C12N 2799/026 where the vector is derived from a baculovirus

Abstract

Disclosed is a retinoic acid receptor epsilon (RARε) polynucleotide and DNA (RNA) encoding such polypeptides. Also provided is a procedure for producing such polypeptide by recombinant techniques and utilizing such polypeptide for therapeutic purposes, for example, tissue regeneration and stimulation of the immune and hematopoietic system. Also disclosed are methods of identifying ligands which stimulate the RARε. Also disclosed are diagnostic methods for detecting a mutation in the RARε receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host.

Claims

1. An isolated polynucleotide comprising a polynucleotide having at least 95% identity to a member selected from the group consisting of:

(a) a polynucleotide encoding a polypeptide comprising amino acid 2 to 433 of SEQ ID NO;

2; and

(b) the complement of (a).

2. The isolated polynucleotide of claim 1 wherein said member is (a).

3. The isolated polynucleotide of claim 1 wherein said member is (a) and the polypeptide comprises amino acids 1 to 433 of SEQ ID NO:

2.

4. The isolated polynucleotide of claim 1 comprising a polynucleotide encoding a polypeptide comprising the amino acid sequence identical to amino acids 2 to 433 of SEQ ID NO:

2.

5. The isolated polynucleotide of claim 1, wherein the polynucleotide is DNA.

6. The isolated polynucleotide of claim 1 comprising a polynucleotide encoding a polypeptide comprising the amino sequence identical to amino acids 1 to 433 of SEQ ID NO:

2.

7. The isolated polynucleotide of claim 1, wherein said polynucleotide is RNA.

8. A method of making a recombinant vector comprising inserting the isolated polynucleotide of claim 2 into a vector, wherein said polynucleotide is DNA.

9. A recombinant vector comprising the polynucleotide of claim 2, wherein said polynucleotide is DNA.

10. A recombinant host cell comprising the polynucleotide of claim 2, wherein said polynucleotide is DNA.

11. A method for producing a polypeptide comprising expressing from the recombinant cell of claim 10 the polypeptide encoded by said polynucleotide and isolated said polypeptide.

12. A process for producing a polypeptide comprising:

expressing from a recombinant cell containing the polynucleotide of claim 4 the polypeptide encoded by said polynucleotide and isolating said polypeptide.

13. A process for producing a polypeptide comprising:

expressing from a recombinant cell containing the polynucleotide of claim 6 the polypeptide encoded by said polynucleotide and isolating said polypeptide.

14. The isolated polynucleotide of claim 4 comprising nucleotides 183 to 1478 of SEQ ID NO:

1.

15. The isolated polynucleotide of claim 4 comprising nucleotides 180 to 1478 of SEQ ID NO:

1.

16. The isolated polynucleotide of claim 4 comprising the nucleotides of the sequence of SEQ ID NO:

1.

17. An isolated polynucleotide comprising the nucleotides of SEQ ID NO:

1 coding for the mature retinoid acid receptor epsilon (RARε

) protein.

18. An isolated polynucleotide comprising a polynucleotide having at least a 95% identity to a member selected from the group consisting of:

(a) a polynucleotide encoding the same mature polypeptide encoded by the human cDNA in ATCC Deposit No. 75754; and

(b) the complement of (a).

19. The isolated polynucleotide of claim 18, wherein the member is (a).

20. The isolated polynucleotide of claim 18, wherein said polynucleotide comprises DNA identical to the coding portion of the human cDNA in ATCC Deposit No 75754 which encodes a mature polypeptide.