Cloning and expression of the gene for bacteriophage T7 RNA polymerase
First Claim
1. An isolated DNA sequence consisting of a cloned T7 RNA polymerase gene encoding an active T7 RNA polymerase.
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Abstract
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
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Citations
5 Claims
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1. An isolated DNA sequence consisting of a cloned T7 RNA polymerase gene encoding an active T7 RNA polymerase.
- 2. A DNA expression vector encoding an active T7 RNA polymerase.
Specification