Method and apparatus for ratiometric measurement of hematocrit

• US 5,869,971 A
• Filed: 05/17/1996
• Issued: 02/09/1999
• Est. Priority Date: 05/17/1996
• Status: Expired due to Fees

• Current Assignee: Radiometer California, Inc. (Danaher Corporation)
• Original Assignee: SenDx Medical, Inc. (Danaher Corporation)
• Inventors: Sherman, Marshall L.
• Primary Examiner(s): BROWN, GLENN WARNER
• Application Number:
  US08/649,525
• Time in Patent Office: 998 Days
• Field of Search: 324/439, 324/442, 324/692, 324/693, 324/71.1
• US Class Current: 324/439
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

A method and apparatus for measuring resistance of a blood sample in order to determine the hematocrit of that blood sample. The present invention makes a direct ratiometric measurement. That is, the alternating current that flows through the sample is directly measured by applying that alternating current to an integrator which converts the current to a D.C. voltage which is directly proportional to the applied current.

Claims

1. A circuit for measuring the conductivity of a blood sample, the circuit including:

a. a first and second connection point, each connection point being configured to be coupled to a blood conductivity sensor and the second connection point also being configured to be coupled to a drive signal source;

b. a half-wave rectifier coupled to the first connection point; and

c. an integrator coupled to the output of the half-wave rectifier.

2. A circuit for measuring the conductivity of a blood sample in order to determine the hematocrit of the blood sample, the circuit including:

a. a first, second, and third connection point, the first and second connection point being configured to be coupled to a first and second electrode of a blood conductivity sensor, the third connection point being configured to be coupled to a drive signal source;

b. a half-wave rectifier coupled to the first connection point;

c. an integrator coupled to the output of the half-wave rectifier; and

d. a filter coupled between the second and third connection point.

3. The circuit of claim 2, wherein an impedance matching circuit is provided between the filter and the third connection point.

4. The circuit of claim 2, wherein an impedance matching circuit is provided between the filter and the second connection point.

5. The circuit of claim 2, wherein a coupling capacitor is provided between the filter and the third connection point.

6. The circuit of claim 2, wherein the filter is a 4-pole filter.

7. The circuit of claim 2, wherein the integrator includes:

(a) an operational amplifier having a non-inverting input, an inverting input, and an output;

(b) a capacitor coupled between the inverting input and the output; and

(c) a resistor coupled between the inverting input and the output in parallel with the capacitor.

8. The circuit of claim 2, wherein the rectifier includes:

a. a first and second switch, each having a signal input, a signal output, and a control input signal, the signal inputs of the first and second switch being coupled together, the output of the first switch being coupled to ground, and the output of the second switch being coupled to the input of the integrator; and

wherein the control input signal to each switch is synchronized to an output drive signal generated by the drive signal source, and the control input signal coupled to the first switch is 180 degrees out-of-phase with the control input signal coupled to the second switch in order to couple the integrator to the hematocrit sensor when current flows in a first direction through the hematocrit sensor and to alternatively disconnect the integrator from the hematocrit sensor and couple the hematocrit sensor to ground when current flows in a second direction through the hematocrit sensor.

9. The circuit of claim 8, wherein a phase difference between the output drive signal and the control input signal coupled to the first switch is essentially equal to the phase shift imposed on a signal after the signal passes through the filter.

10. The circuit of claim 2, wherein a drive signal is provided by the drive signal source.

11. The circuit of claim 10, wherein the drive signal is a 10.8 kHz square wave signal.

12. The circuit of claim 11, wherein the drive signal source divides an 11.0592 MHz signal by 1024 to derive the 10.8 kHz square wave signal.

13. The circuit of claim 12, wherein the drive signal source includes a programmable logic device for performing the division which generates the 10.8 kHz square wave signal.

14. The circuit of claim 2, further including a switch coupled between the filter and the second connection point.

15. The circuit of claim 14, further including a control device coupled to a control input to the switch for generating an enable signal to control operation of the switch.

16. A method for measuring hematocrit of a blood sample, including the steps of:

a. generating an output drive signal;

b. coupling the output drive signal to a first electrode of a blood conductivity sensor;

c. coupling a second electrode of the blood conductivity sensor to a half-wave rectifier;

d. coupling the output from the half-wave rectifier to an integrator;

e. reading the output from the integrator while the blood conductivity electrodes are emersed in a calibrant;

f. reading the output from the integrator while the blood conductivity electrodes are emersed in a blood sample; and

g. determining the hematocrit value of the blood sample by generating a ratio φ

of the integrator output read when the blood conductivity sensor electrodes were emersed in the blood sample with respect to the integrator output read when the blood conductivity sensor electrodes were emersed in the calibrant and applying the ratio φ

to the formula;

space="preserve" listing-type="equation">H=a 1n φ

-b;

where H is the hematocrit value of the blood sample, a is a first known cell constant, and b is a second known cell constant.

17. The method of claim 16, wherein the output drive signal is a square wave signal, and further including the step of shaping the square wave output drive signal into a sinusoidal signal prior to coupling the output drive signal to the blood conductivity sensor.

18. The method of claim 17, further including the step of:

a. synchronizing the half-wave rectifier to the output drive signal such that the second blood conductivity sensor electrode is coupled to the integrator during a negative current phase, and to ground during a positive current phase.