Materials and method for the immobilization of bioactive species onto polymeric subtrates
First Claim
1. A vascular graft having bioactive species immobilized thereto, the vascular graft comprising:
- a porous polytetrafluoroethylene material;
a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the porous polytetrafluoroethylene material and cross-linked to itself in situ;
a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and
at least one type of bioactive species attached to the second layer, the second layer and immobilized bioactive species serving as a blood contact surface.
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Abstract
The present invention is directed to support members having surfaces that are rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto the surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on the support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member, as well as, to provide a substrate for immobilizing bioactive species thereto. Methods for forming the first and second layers are also provided. In addition, methods for attaching bioactive substances to the hydrophilic polymers are provided.
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Citations
14 Claims
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1. A vascular graft having bioactive species immobilized thereto, the vascular graft comprising:
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a porous polytetrafluoroethylene material; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the porous polytetrafluoroethylene material and cross-linked to itself in situ; a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer, the second layer and immobilized bioactive species serving as a blood contact surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A vascular graft having bioactive species immobilized thereto, the vascular graft comprising:
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a porous polytetrafluoroethylene material; a first layer of polyvinyl alcohol non-covalently attached to the porous polytetrafluoroethylene; a second layer of polyvinyl alcohol covalently attached to the first layer; and a bioactive species comprising Gly-Arg-Gly-Asp-Ser attached to the second layer. - View Dependent Claims (13, 14)
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Specification