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Process for sexing cow embryos

  • US 5,876,942 A
  • Filed: 07/24/1997
  • Issued: 03/02/1999
  • Est. Priority Date: 07/24/1997
  • Status: Expired due to Term
First Claim
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1. An oligonucleotide primer set for bovine embryo sexing wherein said oligonucleotide primer set comprises a primer pair which primer pair consists of individual oligonucleotide primers each of which is able to hybridize specifically and simultaneously to an intron 5 sequence of the bovine amelogenin gene, which gene is located on both the bovine X and Y chromosomes, wherein said intron 5 sequence of the bovine amelogenin gene is selected from the group consisting of:

  • SEQ ID NO;

    21 (the nucleotide sequence of intron 5 of the bovine amelogenin gene located on the bovine X chromosome), that sequence fully complementary to SEQ ID NO;

    21, SEQ ID NO;

    22 (the nucleotide sequence of intron 5 of the bovine amelogenin gene located on the bovine Y chromosome), and that sequence fully complementary to SEQ ID NO;

    22.

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