Thermostable muts proteins

• US 5,877,280 A
• Filed: 06/06/1995
• Issued: 03/02/1999
• Est. Priority Date: 06/06/1995
• Status: Expired due to Term

• Current Assignee: The Mount Sinai Medical Center of The City University of New York
• Original Assignee: Mount Sinai School of Medicine of New York University
• Inventors: Wetmur, James G.
• Primary Examiner(s): Marschel, Ardin H.
• Assistant Examiner(s): Riley, Jezia
• Application Number:
  US08/468,558
• Time in Patent Office: 1,365 Days
• Field of Search: 435/6, 435/91.1, 436/501, 436/94, 935/77, 935/78, 530/350
• US Class Current: 530/350
• CPC Class Codes: C07K 14/195 from bacteria
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Isolated nucleic acids which encode a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid and recombinant vectors comprising nucleic acid which encodes a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid are disclosed. Also disclosed are isolated thermostable proteins which bind specifically to bulge loops in a heteroduplex nucleic acid and host cells comprising a recombinant gene which can express a thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid. Further disclosed are a method of reducing DNA misincorporation in an amplification reaction, methods for detecting a nucleic acid which includes a specific sequence, a method for amplifying a nucleic acid comprising a specific sequence, and a method for selecting against a nucleic acid comprising a specific sequence.

Claims

1. Isolated MutS obtained from bacteria selected from the group consisting of hyperthermophilic bacteria and thermophilic bacteria.

2. An isolated thermostable protein consisting of the contiguous amino acid sequence of SEQ ID NO:

2.

3. An isolated thermostable protein consisting of the contiguous amino acid sequence of SEQ ID NO:

5.

4. An isolated thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid encoded by a nucleic acid characterized by the ability to hybridize under high stringency conditions to DNA consisting of the contiguous sequence of SEQ ID NO:

6.

5. An isolated thermostable protein which binds specifically to bulge loops in a heteroduplex nucleic acid encoded by a nucleic acid characterized by the ability to hybridize under high stringency conditions to DNA consisting of the contiguous sequence of SEQ ID NO:

7.